. Transneuronal transport of neurotropic viruses is uniquely able to reveal the cellular composition of multi-synaptic circuits. As a consequence, this technique is providing fundamental new insights into the functional architecture of sensory, motor, cognitive and affective networks in the central nervous system. The Center for Neuroanatomy with Neurotropic Viruses (CNNV) was created in 2004 to provide the neuroscience community with access to the highly specialized reagents, training and facilities that are necessary to use neurotropic viruses as transneuronal tracers. We document in this application that the CNNV has become an essential national and international resource for an important segment of the neuroscience community. Users of the Center are funded by 15 different institutes of the NIH, by NSF and by private foundations as well as international research organizations. The Center has established a strong history of providing: 1) well-characterized reagents, 2) training and guidance in the use of the method, and 3) specialized biohazard facilities for performing experiments using neurotropic viruses. The Center also has played a vital role in the development of new and improved transneuronal tracing tools that permit more targeted dissection of the functional architecture of neural circuits. Furthermore, the Center has served as a critical technical and intellectual resource for those interested in using this method. The goal of this competitive renewal is to enable the Center to continue both its resource and research functions.

Public Health Relevance

The ability to treat disease and injury of the brain depends upon developing a fundamental understanding of the complex neural circuits that comprise the central nervous system. The use of neurotrophic viruses as transneuronal tracers provides a unique and powerful tool that reveals the functional organization of these complex circuits. The CNNV is a national resource that provides training in the use of this approach and distributes reagents to a world-wide cadre of investigators.

Agency
National Institute of Health (NIH)
Institute
Office of The Director, National Institutes of Health (OD)
Type
Animal (Mammalian and Nonmammalian) Model, and Animal and Biological Material Resource Grants (P40)
Project #
2P40OD010996-16
Application #
10023321
Study Section
Special Emphasis Panel (ZRG1)
Program Officer
Watson, Harold L
Project Start
2003-07-01
Project End
2025-06-30
Budget Start
2020-07-01
Budget End
2021-06-30
Support Year
16
Fiscal Year
2020
Total Cost
Indirect Cost
Name
University of Pittsburgh
Department
Biology
Type
Schools of Medicine
DUNS #
004514360
City
Pittsburgh
State
PA
Country
United States
Zip Code
15260
Guest, Jason M; Seetharama, Mythreya M; Wendel, Elizabeth S et al. (2018) 3D reconstruction and standardization of the rat facial nucleus for precise mapping of vibrissal motor networks. Neuroscience 368:171-186
Bostan, Andreea C; Strick, Peter L (2018) The basal ganglia and the cerebellum: nodes in an integrated network. Nat Rev Neurosci 19:338-350
Krieger, Jean-Philippe; Santos da Conceição, Ellen Paula; Sanchez-Watts, Graciela et al. (2018) Glucagon-like peptide-1 regulates brown adipose tissue thermogenesis via the gut-brain axis in rats. Am J Physiol Regul Integr Comp Physiol 315:R708-R720
Wang, Desheng; Smith-Bell, Carrie A; Burhans, Lauren B et al. (2018) Changes in membrane properties of rat deep cerebellar nuclear projection neurons during acquisition of eyeblink conditioning. Proc Natl Acad Sci U S A 115:E9419-E9428
MacGibeny, Margaret A; Koyuncu, Orkide O; Wirblich, Christoph et al. (2018) Retrograde axonal transport of rabies virus is unaffected by interferon treatment but blocked by emetine locally in axons. PLoS Pathog 14:e1007188
Meyers, Eric C; Solorzano, Bleyda R; James, Justin et al. (2018) Vagus Nerve Stimulation Enhances Stable Plasticity and Generalization of Stroke Recovery. Stroke 49:710-717
Gao, Hong; Molinas, Adrien J R; Miyata, Kayoko et al. (2017) Overactivity of Liver-Related Neurons in the Paraventricular Nucleus of the Hypothalamus: Electrophysiological Findings in db/db Mice. J Neurosci 37:11140-11150
Watson, Alan M; Rose, Annika H; Gibson, Gregory A et al. (2017) Ribbon scanning confocal for high-speed high-resolution volume imaging of brain. PLoS One 12:e0180486
Rathelot, Jean-Alban; Dum, Richard P; Strick, Peter L (2017) Posterior parietal cortex contains a command apparatus for hand movements. Proc Natl Acad Sci U S A 114:4255-4260
Caligiore, Daniele; Pezzulo, Giovanni; Baldassarre, Gianluca et al. (2017) Consensus Paper: Towards a Systems-Level View of Cerebellar Function: the Interplay Between Cerebellum, Basal Ganglia, and Cortex. Cerebellum 16:203-229

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