Zebrafish is a premiere organism to study vertebrate development, physiology, behavior, genetics and disease. Powerful techniques allow efficient generation and recovery of zebrafish mutations affecting genes that regulate developmental patterning, organogenesis, physiology and behavior. Recent advances make it easy to study gene function in transgenic zebrafish and with antisense oligonucleotides. The functions of many, if not most of these genes are conserved among vertebrate groups. Thus, analysis of zebrafish mutations provides insights into gene function in other vertebrates, including humans. The Zebrafish International Resource Center has been established as a repository that provides animals, materials and services to the research community. This proposal seeks continued funding 1) to provide a centralized repository for zebrafish genetic stocks and research materials, 2) to provide consultation and pathology services, and 3) to develop improved zebrafish husbandry methods. These materials, services and information will be made widely available to the research community.

Public Health Relevance

The Zebrafish International Resource Genter 1) provides a centralized repository for zebrafish genetic stocks and research materials, 2) provides consultation and pathology services, and 3) develops improved zebrafish husbandry methods. These materials, services and information will be made widely available to the research community.

Agency
National Institute of Health (NIH)
Institute
Office of The Director, National Institutes of Health (OD)
Type
Animal (Mammalian and Nonmammalian) Model, and Animal and Biological Material Resource Grants (P40)
Project #
2P40OD011021-16
Application #
8552199
Study Section
Special Emphasis Panel (ZOD1)
Program Officer
Contreras, Miguel A
Project Start
1998-05-01
Project End
2018-04-30
Budget Start
2014-05-01
Budget End
2015-04-30
Support Year
16
Fiscal Year
2014
Total Cost
Indirect Cost
Name
University of Oregon
Department
Neurosciences
Type
Graduate Schools
DUNS #
City
Eugene
State
OR
Country
United States
Zip Code
97403
Matthews, Jennifer L; Murphy, Joy M; Carmichael, Carrie et al. (2018) Changes to Extender, Cryoprotective Medium, and In Vitro Fertilization Improve Zebrafish Sperm Cryopreservation. Zebrafish 15:279-290
Gonzalez, Trevor J; Lu, Yuan; Boswell, Mikki et al. (2018) Fluorescent light exposure incites acute and prolonged immune responses in zebrafish (Danio rerio) skin. Comp Biochem Physiol C Toxicol Pharmacol 208:87-95
Murray, Katrina N; Wolf, Jeffrey C; Spagnoli, Sean T et al. (2018) Reversibility of Proliferative Thyroid Lesions Induced by Iodine Deficiency in a Laboratory Zebrafish Colony. Zebrafish 15:558-565
Spagnoli, S; Sanders, J; Kent, M L (2017) The common neural parasite Pseudoloma neurophilia causes altered shoaling behaviour in adult laboratory zebrafish (Danio rerio) and its implications for neurobehavioural research. J Fish Dis 40:443-446
Köhler, Almut; Collymore, Chereen; Finger-Baier, Karin et al. (2017) Report of Workshop on Euthanasia for Zebrafish-A Matter of Welfare and Science. Zebrafish 14:547-551
Wilson, Alexandria K; Watral, Virginia G; Kent, Michael L et al. (2017) Draft Genome Sequence of Pseudomonas sp. Strain DrBHI1 (Phylum Proteobacteria). Genome Announc 5:
Meritet, Danielle M; Mulrooney, Donna M; Kent, Michael L et al. (2017) Development of Quantitative Real-Time PCR Assays for Postmortem Detection of Mycobacterium spp. Common in Zebrafish (Danio rerio) Research Colonies. J Am Assoc Lab Anim Sci 56:131-141
Samaee, Seyed-Mohammadreza; Seyedin, Sahar; Varga, Zoltan M (2017) An Affordable Intraperitoneal Injection Setup for Juvenile and Adult Zebrafish. Zebrafish 14:77-79
Martins, M L; Watral, V; Rodrigues-Soares, J P et al. (2017) A method for collecting eggs of Pseudocapillaria tomentosa (Nematoda: Capillariidae) from zebrafish Danio rerio and efficacy of heat and chlorine for killing the nematode's eggs. J Fish Dis 40:169-182
Varga, Zoltán M; Wilson, Carole; Alestrøm, Peter (2016) European Zebrafish Meeting 2015 Husbandry Session Report. Zebrafish 13:230-1

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