Abstract

This component addresses the significance and need to develop a protocol for cryopreserving axolotl germplasm. The AGSC will work with the Aquatic Germplasm and Genetics Research Center at Louisiana State University (LSU) to develop a reproducible protocol for cryopreserving axolotl sperm. Early results are presented to show that the AGSC has already initiated collaborative studies with the AGGRC to assess axolotl sperm viability and motility after overnight transport from Lexington to Louisiana. Building upon this work, the AGSC will ship axolotl sperm to the AGGRC to identify optimal conditions for cryopreservation. After a working protocol has been established, AGSC staff will visit the AGGRC to learn how to assess sperm quality and perform the cryopreservation procedure. Additionally, AGGRC staff will help the AGSC develop a repository strategy for community-wide management of axolotl sperm. Upon returning to Lexington, KY, AGSC staff will implement the protocol, examine the viability of frozen sperm, and then use cryopreserved sperm to re-derive axolotl stocks. Accomplishment of this objective will allow for more efficient management of axolotl stocks within the AGSC and the broader axolotl community

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
Office of The Director, National Institutes of Health (OD)
Type
Animal (Mammalian and Nonmammalian) Model, and Animal and Biological Material Resource Grants (P40)
Project #
2P40OD019794-06
Application #
9853492
Study Section
Special Emphasis Panel (ZRG1)
Project Start
Project End
Budget Start
2020-03-01
Budget End
2021-02-28
Support Year
6
Fiscal Year
2020
Total Cost
Indirect Cost

  Institution

Name
University of Kentucky
Department
Type
DUNS #
939017877
City
Lexington
State
KY
Country
United States
Zip Code
40526

  Publications

Al Haj Baddar, Nour W; Chithrala, Adarsh; Voss, S Randal (2018) Amputation-induced reactive oxygen species signaling is required for axolotl tail regeneration. Dev Dyn :
Randal Voss, S; Murrugarra, David; Jensen, Tyler B et al. (2018) Transcriptional correlates of proximal-distal identify and regeneration timing in axolotl limbs. Comp Biochem Physiol C Toxicol Pharmacol 208:53-63
Johnson, Kimberly; Bateman, Joel; DiTommaso, Tia et al. (2018) Systemic cell cycle activation is induced following complex tissue injury in axolotl. Dev Biol 433:461-472
Franklin, Brandon M; Voss, S Randal; Osborn, Jeffrey L (2017) Ion channel signaling influences cellular proliferation and phagocyte activity during axolotl tail regeneration. Mech Dev 146:42-54
Bryant, Donald M; Johnson, Kimberly; DiTommaso, Tia et al. (2017) A Tissue-Mapped Axolotl De Novo Transcriptome Enables Identification of Limb Regeneration Factors. Cell Rep 18:762-776
Ritenour, Angela M; Dickie, Renee (2017) Inhibition of Vascular Endothelial Growth Factor Receptor Decreases Regenerative Angiogenesis in Axolotls. Anat Rec (Hoboken) 300:2273-2280
Woodcock, M Ryan; Vaughn-Wolfe, Jennifer; Elias, Alexandra et al. (2017) Identification of Mutant Genes and Introgressed Tiger Salamander DNA in the Laboratory Axolotl, Ambystoma mexicanum. Sci Rep 7:6
Montoro, Rodrigo; Dickie, Renee (2017) Comparison of tissue processing methods for microvascular visualization in axolotls. MethodsX 4:265-273
McCusker, Catherine D; Diaz-Castillo, Carlos; Sosnik, Julian et al. (2016) Cartilage and bone cells do not participate in skeletal regeneration in Ambystoma mexicanum limbs. Dev Biol 416:26-33
Keinath, Melissa C; Timoshevskiy, Vladimir A; Timoshevskaya, Nataliya Y et al. (2015) Initial characterization of the large genome of the salamander Ambystoma mexicanum using shotgun and laser capture chromosome sequencing. Sci Rep 5:16413

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