The specific aims of this proposal are to continue the development of a bank of frozen embryos obtained from valuable genetic stocks maintained at the Jackson Laboratory; to determine the importance of environmental or seasonal effects on the ability to superovulate mice representing different genotypes; to determine the importance of the genetic relationship between embryo and pseudpregnant foster mother upon embryo transfer; and to determine the importance of seasonal environmental variations on the rate of live-born recovery after embryo transfer. Eight-cell embryos will be collected from superovulated female donors, frozen and stored in liquid nitrogen. At least 1,000 embryos from each of over 400 different stocks will be stored in two separate liquid nitrogen refrigerators. Breeding pairs of mice recovered from frozen storage will be provided at cost to qualified investigators when such mice are not elsewhere available. The long-term objective of this project is to establish a bank of frozen embryos so as to provide assurance of the preservation of mutants and strains of mice that are valuable to many fields of research including genetics, cancer, immunology, development and reproduction, aging, blood research, behavior, and neurobiology. The principal purpose of this frozen embryo bank is to preserve irreplaceable murine germplasm, but other beneficial purposes are to reduce the necessary number of different stocks or size of colonies maintained by conventional breeding procedures and to retard genetic drift.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Animal (Mammalian and Nonmammalian) Model, and Animal and Biological Material Resource Grants (P40)
Project #
5P40RR001262-09
Application #
3103427
Study Section
Human Embryology and Development Subcommittee 1 (HED)
Project Start
1982-05-01
Project End
1992-04-30
Budget Start
1990-05-01
Budget End
1991-04-30
Support Year
9
Fiscal Year
1990
Total Cost
Indirect Cost
Name
Jackson Laboratory
Department
Type
DUNS #
042140483
City
Bar Harbor
State
ME
Country
United States
Zip Code
04609
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