The yeast Genetic Stock Center has been in operation since the early 1960's and has been officially funded by the NSF since 1972. The YGSC maintains and disseminates strains of S. cerevisiae for use in basic and applied research in the biological sciences and for teaching purposes. The collection contains about 850 genetically well-characterized strains, which are periodically checked for markers and ploidy. Improved versions of particular strains are occassionally constructed by the YGSC, preserving as much as possible of the original genome. Completely now constructs (such as opposite mating type or limited additional auxotrophies) for certain mutants are periodically made and added to the collection. Well characterized new strains of general interest and usefulness are solicited from, or deposited by, various investigators, and, once checked, are incorporated into the collection. At intervals of 2 of 3 years, the YGSC publishes and distributes to more than 1000 investigators and educators a catalogue listing all strains in the collection, their genotypes, sources and pertinent references. Approximately 100 strains are requested and supplied each year. This year, requests have already exceeded 1000 strains. New techniques have recently been adapted for use in better characterizing strains in the collection. Orthogonal Field Alteration Gel Electrophoresis (OFAGE), in conjunction with DNA-DNA hybridization, is being implemented by the YGSC for the karyotyping of various """"""""key"""""""" strains of S. cerevisiae and other yeast genera, as well as for the identification of chromosomal anomalies (translocations, rearrangements and other ploidy phenomena) in certain strains in the collection.

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University of California Berkeley
Schools of Arts and Sciences
United States
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Basile, G; Aker, M; Mortimer, R K (1992) Nucleotide sequence and transcriptional regulation of the yeast recombinational repair gene RAD51. Mol Cell Biol 12:3235-46
Mortimer, R K; Contopoulou, C R; King, J S (1992) Genetic and physical maps of Saccharomyces cerevisiae, Edition 11. Yeast 8:817-902
Kans, J A; Mortimer, R K (1991) Nucleotide sequence of the RAD57 gene of Saccharomyces cerevisiae. Gene 105:139-40
King, J S; Mortimer, R K (1991) A mathematical model of interference for use in constructing linkage maps from tetrad data. Genetics 129:597-602
Cook, V E; Mortimer, R K (1991) A quantitative model of DNA fragments generated by ionizing radiation, and possible experimental applications. Radiat Res 125:102-6
King, J S; Mortimer, R K (1990) A polymerization model of chiasma interference and corresponding computer simulation. Genetics 126:1127-38
Mortimer, R K; Schild, D; Contopoulou, C R et al. (1989) Genetic map of Saccharomyces cerevisiae, edition 10. Yeast 5:321-403