This competing renewal seeks continued funding for The Jackson Laboratory's (TJL) Induced Mutant Resource (IMR) to continue to provide this critical resource of transgenic and targeted mutation mice to the scientific community. Selectively altering the mouse genome by gene transfer (transgenic mice), homologous recombination and temporal- and tissue-specific production and use of induced mutant mice generated by these means has rapidly expanded, increasing the need for a resource to maintain, preserve, and distribute these mutant mice at a high health status and with minimal restrictions. Researchers frequently receive requests for mice they have created and want to believed of the burden that distributing mice to other scientists places on their research programs and grants. TJL, founded in 1929 as a non-profit research institution, has maintained and distributed mutant strains of mice for nearly 50 years. In 1992, TJL established the IMR whose purpose is to import, cryopreserve, and distribute biomedically important induced mutant mice. The need for the IMR is compelling and evidence by its rapid growth. Since it began, the IMR has accepted 495 mutant stocks, is distributing 299 of these, with another 58 currently in the importation process. In 1997, nearly 60,000 mice were distributed. We request support for rederivation, cryopreservation, and strain development, and strain development to improve the scientific value of strains in the IMR; mice are maintained for distribution to other scientists on as much of a cost recovery basis as possible. To improve the IMR resource program we propose to do research to: (1) develop methods to assure pathogen-free cyropreservation of mouse sperm, (2) phenotypically characterize new induced mutations and mutations transferred to new congenic genetic backgrounds, and (3) develop more cost-effective genotyping methods. Demand for IMR mice continues to increase, coincident with a decline in funding as several non-renewable start-up grants terminate. The ratio of rejected to accepted strains is increasing (see Introduction). The size of the need is beyond the scope of the NCRR alone to fund. NIAID and NIAMS already contribute to this effort by Intra-Agency Agreements. During the first 4 years of the current grant, over 70% of the mice supplied by the IMR were received by NIH intramural and extramural grantees of 16 NIH Institutes. To address the growing need we request approval of an expansion of this national resource for transgenic and targeted mice to authorize the NCRR to seek additional funding from NIH institutes. To aid in review we have subdivided the overall budget components. The Introduction explains the current funding mechanisms and our proposal to expand the IMR without an excessive burden to the NCRR.

National Institute of Health (NIH)
National Center for Research Resources (NCRR)
Animal (Mammalian and Nonmammalian) Model, and Animal and Biological Material Resource Grants (P40)
Project #
Application #
Study Section
National Center for Research Resources Initial Review Group (RIRG)
Program Officer
Grieder, Franziska B
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Jackson Laboratory
Bar Harbor
United States
Zip Code
Leiter, Edward H; Reifsnyder, Peter C; Wallace, Racheal et al. (2009) NOD x 129.H2(g7) backcross delineates 129S1/SvImJ-derived genomic regions modulating type 1 diabetes development in mice. Diabetes 58:1700-3
Davisson, Muriel T; Taft, Robert A (2006) Strategies for managing an ever increasing mutant mouse repository. Brain Res 1091:255-7
Petkov, Petko M; Cassell, Megan A; Sargent, Evelyn E et al. (2004) Development of a SNP genotyping panel for genetic monitoring of the laboratory mouse. Genomics 83:902-11
Sztein, J M; Noble, K; Farley, J S et al. (2001) Comparison of permeating and nonpermeating cryoprotectants for mouse sperm cryopreservation. Cryobiology 42:28-39
Sharp, J J; Linder, C C; Mobraaten, L E (2001) Genetically engineered mice. Husbandry and resources. Methods Mol Biol 158:381-96
Sztein, J M; Farley, J S; Mobraaten, L E (2000) In vitro fertilization with cryopreserved inbred mouse sperm. Biol Reprod 63:1774-80
Reifsnyder, P C; Flynn, J C; Gavin, A L et al. (1999) Genotypic and phenotypic characterization of six new recombinant congenic strains derived from NOD/Shi and CBA/J genomes. Mamm Genome 10:161-7
Sztein, J M; McGregor, T E; Bedigian, H J et al. (1999) Transgenic mouse strain rescue by frozen ovaries. Lab Anim Sci 49:99-100
Sztein, J; Sweet, H; Farley, J et al. (1998) Cryopreservation and orthotopic transplantation of mouse ovaries: new approach in gamete banking. Biol Reprod 58:1071-4
Sztein, J M; Farley, J S; Young, A F et al. (1997) Motility of cryopreserved mouse spermatozoa affected by temperature of collection and rate of thawing. Cryobiology 35:46-52

Showing the most recent 10 out of 12 publications