This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): This proposal describes the continuation of the National Resource for Aplysia, whose overall goal is to provide consistently high-quality cultured sea hares Aplysia californica (and their cultured red algal food, Gracilaria sp.) to NIH-sponsored researchers. Aplysia californica is an important non-vertebrate (Opisthobranch mollusc) model system for health-related research, primarily in the neurophysiology of behavior and learning. We will continue to produce animals for research, and to conduct basic research aimed at exploring new model uses and at improving the resource. There are four sub-projects/specific aims: 1. Production-We anticipate increasing our production of animals by approximately 25% per year during this next phase of funding and will make specimens from all life stages available at a price competitive with fieldcollected specimens. 2. Functional Genomics-We will undertake a cDNA/EST sequencing project of neural and other tissues from different developmental stages (larvae, juveniles, and reproductive and senescent animals), and use these cDNAs to produce microarrays. Following appropriate validation and quality control, these microarrays will be available for at-cost purchase by our user community, and will also be used to examine gene expression changes in our hatchery population during different developmental stages, and after exposure to other experimental conditions including variable temperature, egg-laying hormone administration, etc. All sequence and array experimentation data will be made available through web-based database access. 3. Developmental Neurophysiology- We will continue study of ion current modulation in the bag cells observed to vary with development, genetic background, and between hatchery reared and wild caught animals. Understanding electrophysiological correlates of growth and maturation are important to the Resource because they may affect the interpretation of data by users of the Resource. A correlative study will examine how different growth rates of the animals produce variations in development times for the nervous system changes that culminate in sexual maturity. 4. Animal Health Monitoring-We will continue a monitoring program based on screening of water quality and animal health parameters to assure rapid detection and complete documentation of any disease processes that might occur in any developmental stages of animals at the hatchery. Any disease syndromes and suspected pathogens observed will be investigated and appropriate control measures applied. Through this combination of production and basic research, we will improve the model system and extend its usefullness to other areas of research.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Animal (Mammalian and Nonmammalian) Model, and Animal and Biological Material Resource Grants (P40)
Project #
5P40RR010294-11
Application #
7392004
Study Section
Special Emphasis Panel (ZRR1-CM-2 (01))
Project Start
2006-06-01
Project End
2007-05-31
Budget Start
2006-06-01
Budget End
2007-05-31
Support Year
11
Fiscal Year
2006
Total Cost
$547,377
Indirect Cost
Name
University of Miami Rosenteil School
Department
Type
DUNS #
152764007
City
Miami
State
FL
Country
United States
Zip Code
33149
Hu, Jiangyuan; Adler, Kerry; Farah, Carole Abi et al. (2017) Cell-Specific PKM Isoforms Contribute to the Maintenance of Different Forms of Persistent Long-Term Synaptic Plasticity. J Neurosci 37:2746-2763
Hu, Jiangyuan; Ferguson, Larissa; Adler, Kerry et al. (2017) Selective Erasure of Distinct Forms of Long-Term Synaptic Plasticity Underlying Different Forms of Memory in the Same Postsynaptic Neuron. Curr Biol 27:1888-1899.e4
Jarvis, Tayler A; Capo, Thomas R; Bielmyer-Fraser, Gretchen K (2015) Dietary metal toxicity to the marine sea hare, Aplysia californica. Comp Biochem Physiol C Toxicol Pharmacol 174-175:54-64
Hu, Jiang-Yuan; Levine, Amir; Sung, Ying-Ju et al. (2015) cJun and CREB2 in the postsynaptic neuron contribute to persistent long-term facilitation at a behaviorally relevant synapse. J Neurosci 35:386-95
Hu, Jiangyuan; Schacher, Samuel (2015) Persistent Associative Plasticity at an Identified Synapse Underlying Classical Conditioning Becomes Labile with Short-Term Homosynaptic Activation. J Neurosci 35:16159-70
Hu, Jiang-Yuan; Schacher, Samuel (2014) Persistent long-term facilitation at an identified synapse becomes labile with activation of short-term heterosynaptic plasticity. J Neurosci 34:4776-85
Lee, Chang Young; Romanova, Elena V; Sweedler, Jonathan V (2013) Laminar stream of detergents for subcellular neurite damage in a microfluidic device: a simple tool for the study of neuroregeneration. J Neural Eng 10:036020
Ludwar, Bjoern Ch; Evans, Colin G; Cropper, Elizabeth C (2012) Monitoring changes in the intracellular calcium concentration and synaptic efficacy in the mollusc Aplysia. J Vis Exp :e3907
Albright, Rebecca; Bland, Charnelle; Gillette, Phillip et al. (2012) Juvenile growth of the tropical sea urchin Lytechinus variegatus exposed to near-future ocean acidification scenarios. J Exp Mar Bio Ecol 426-427:12-17
Romanova, Elena V; Sasaki, Kosei; Alexeeva, Vera et al. (2012) Urotensin II in invertebrates: from structure to function in Aplysia californica. PLoS One 7:e48764

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