This is a competing renewal application for a Center to prepare adult stem/progenitor cells from the bone marrow of human volunteers and rodents, define the quality of the cells, and then distribute them to multiple investigators. The adult stem/progenitor cells we prepare were originally referred to as fibroblastic colony forming - units, then as marrow stromal cells in the hematological literature, subsequently as mesenchymal stem cells, and most recently as multipotent mesenchymal stromal cells or MSCs. Since its establishment in June of 2003, the Center has made 451 shipments of 1,013 vials of frozen MSCs to 220 individual investigators, 129 of which are NIH funded (Appendix A). An additional 41 investigators have submitted MTAs to receive MSCs;of which 14 are NIH funded. A total of 114 investigators have provided letters of support for this competing renewal application.
The Specific Aims are: 1. To continue to produce standardized preparations of human MSCs (hMSCs) for distribution to other investigators. 2. To continue to provide similarly characterized preparations of rat MSCs (rMSCs) for distribution to other investigators. 3. To continue to provide similar preparations of mouse MSCs (moMSCs) for distribution to other investigators. 4. To develop improved methods for isolating and characterizing hMSCs. The need for experiments with fully characterized MSCs has become critical with the current efforts by us and others to initiate Phase I and Phase II clinical trials with MSCs.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Animal (Mammalian and Nonmammalian) Model, and Animal and Biological Material Resource Grants (P40)
Project #
5P40RR017447-07
Application #
7691777
Study Section
National Center for Research Resources Initial Review Group (RIRG)
Program Officer
Harding, John D
Project Start
2003-06-01
Project End
2013-05-31
Budget Start
2009-06-01
Budget End
2010-05-31
Support Year
7
Fiscal Year
2009
Total Cost
$1,046,221
Indirect Cost
Name
Texas A&M University
Department
Type
Schools of Medicine
DUNS #
835607441
City
College Station
State
TX
Country
United States
Zip Code
77845
Lei, Jennifer; Murphy, William L; Temenoff, Johnna S (2018) Combination of Heparin Binding Peptide and Heparin Cell Surface Coatings for Mesenchymal Stem Cell Spheroid Assembly. Bioconjug Chem 29:878-884
Criscenti, Giuseppe; De Maria, Carmelo; Longoni, Alessia et al. (2018) Soft-molecular imprinted electrospun scaffolds to mimic specific biological tissues. Biofabrication 10:045005
Baek, Jihye; Sovani, Sujata; Choi, Wonchul et al. (2018) Meniscal Tissue Engineering Using Aligned Collagen Fibrous Scaffolds: Comparison of Different Human Cell Sources. Tissue Eng Part A 24:81-93
Betancourt, Beatriz A Pazmino; Florczyk, Stephen J; Simon, Mylene et al. (2018) Effect of the scaffold microenvironment on cell polarizability and capacitance determined by probabilistic computations. Biomed Mater 13:025012
Whitely, Michael; Cereceres, Stacy; Dhavalikar, Prachi et al. (2018) Improved in situ seeding of 3D printed scaffolds using cell-releasing hydrogels. Biomaterials 185:194-204
Takeda, Katsuyuki; Ning, Fangkun; Domenico, Joanne et al. (2018) Activation of p70S6 Kinase-1 in Mesenchymal Stem Cells Is Essential to Lung Tissue Repair. Stem Cells Transl Med 7:551-558
Navarro-Requena, Claudia; Weaver, Jessica D; Clark, Amy Y et al. (2018) PEG hydrogel containing calcium-releasing particles and mesenchymal stromal cells promote vessel maturation. Acta Biomater 67:53-65
Shigemoto-Kuroda, Taeko; Oh, Joo Youn; Kim, Dong-Ki et al. (2017) MSC-derived Extracellular Vesicles Attenuate Immune Responses in Two Autoimmune Murine Models: Type 1 Diabetes and Uveoretinitis. Stem Cell Reports 8:1214-1225
Bajcsy, Peter; Yoon, Soweon; Florczyk, Stephen J et al. (2017) Modeling, validation and verification of three-dimensional cell-scaffold contacts from terabyte-sized images. BMC Bioinformatics 18:526
Furtos, Gabriel; Rivero, Guadalupe; Rapuntean, Sorin et al. (2017) Amoxicillin-loaded electrospun nanocomposite membranes for dental applications. J Biomed Mater Res B Appl Biomater 105:966-976

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