The Viper Resource Center (VRC), P40 competitive renewal, at Texas A&M University-Kingsville proposes to continue the aims of the initial grant application. The resources will be expanded and snake venom, venom fractions, blood and cells will be made available to the biomedical research community. The research segment of the proposal will focus on improving the products by characterizing important molecules found in venom for biomedical research. Because of university support and NIH funding, the VRC within the Natural Toxins Research Center (NTRC) has developed a unique facility with the state-of-the-art instrumentation. There are 300 different species of venomous snakes in the world and venoms are complex mixtures of thousands of molecules. Many venomous snakes use their venoms for capturing prey by interfering with the clotting cascade, which causes the animal to bleed to death. These same molecules could have therapeutic value for preventing heart attacks and strokes. A good example of these molecules is the disintegrins, which are competitive inhibitors of fibrinogen and interfere with platelet function. Disintegrins block cell-to-cell, cell-to-matrix interactions and signal transduction, thereby affecting the internal and external cellular activities of many different cells. It is our hypothesis that snake venoms are abundant and stable sources of many molecules and once purified, characterized, and cloned, could have many potential applications in medicine. Based on this hypothesis, the specific aims are to continue: (1) increasing the number of venomous snakes at the VRC for biomedical use;(2) characterizing snake venoms by high performance liquid chromatography and various assays to determine medical applications of venom molecules;(3) improving the Internet database for better dissemination of information to investigators;and (4) using the latest techniques such as proteomics to determine the molecular weights and amino acid sequences of venom proteins. The expected results will be that investigators will have access to a wide variety of properly maintained snakes, quality venoms and characterized fractions for biomedical research. The snakes, their venoms and venom fractions are placed on the NTRC Internet database, which can be queried. Venom can be ordered at a later date from the same snake, since all snakes have pit tags. Evaluation of the VRC will be based on the number of investigators using the venoms, publications, and information disseminated through the Internet database. The significance of the proposed research is that venom molecules have important biomedical applications in many different areas such as strokes, heart attacks, cancer and other medical problems.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Animal (Mammalian and Nonmammalian) Model, and Animal and Biological Material Resource Grants (P40)
Project #
5P40RR018300-09
Application #
8062072
Study Section
National Center for Research Resources Initial Review Group (RIRG)
Program Officer
Chang, Michael
Project Start
2003-04-15
Project End
2013-04-30
Budget Start
2011-05-01
Budget End
2012-04-30
Support Year
9
Fiscal Year
2011
Total Cost
$489,552
Indirect Cost
Name
Texas A&M University-Kingsville
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
868154089
City
Kingsville
State
TX
Country
United States
Zip Code
78363
Ramos, Carla J; Gutierrez, Daniel A; Aranda, Ana S et al. (2016) Functional characterization of six aspartate (D) recombinant mojastin mutants (r-Moj): A second aspartate amino acid carboxyl to the RGD in r-Moj-D_ peptides is not sufficient to induce apoptosis of SK-Mel-28 cells. Toxicon 118:36-42
Gutierrez, Daniel A; Aranda, Ana S; Carrillo, David A R et al. (2016) Functional analysis of four single (RGDWL, RGDWM, RGDWP, RGDMN) and two double (RGDNM, RGDMP) mutants: The importance of methionine (M) in the functional potency of recombinant mojastin (r-Moj). Toxicon 124:1-7
Giron, Maria E; Rodriguez-Acosta, Alexis; Salazar, Ana Maria et al. (2013) Isolation and characterization of two new non-hemorrhagic metalloproteinases with fibrinogenolytic activity from the mapanare (Bothrops colombiensis) venom. Arch Toxicol 87:197-208
Carey, Clayton M; Bueno, Raymund; Gutierrez, Daniel A et al. (2012) Recombinant rubistatin (r-Rub), an MVD disintegrin, inhibits cell migration and proliferation, and is a strong apoptotic inducer of the human melanoma cell line SK-Mel-28. Toxicon 59:241-8
Calvete, Juan J; Pérez, Alicia; Lomonte, Bruno et al. (2012) Snake venomics of Crotalus tigris: the minimalist toxin arsenal of the deadliest Nearctic rattlesnake venom. Evolutionary Clues for generating a pan-specific antivenom against crotalid type II venoms [corrected]. J Proteome Res 11:1382-90
Lucena, Sara E; Jia, Ying; Soto, Julio G et al. (2012) Anti-invasive and anti-adhesive activities of a recombinant disintegrin, r-viridistatin 2, derived from the Prairie rattlesnake (Crotalus viridis viridis). Toxicon 60:31-9
Yin, Chunhui; Jia, Ying; Garcia, Carlos A (2012) A novel method for the purification of low soluble recombinant C-type lectin proteins. Biochem Biophys Res Commun 425:636-41
Massey, Daniel J; Calvete, Juan J; Sánchez, Elda E et al. (2012) Venom variability and envenoming severity outcomes of the Crotalus scutulatus scutulatus (Mojave rattlesnake) from Southern Arizona. J Proteomics 75:2576-87
Sanchez, Elda E; Hotle, Doug; Rodriguez-Acosta, Alexis (2011) Neutralization of Bitis parviocula (Ethiopian mountain adder) venom by the South African Institute of Medical Research (SAIMR) antivenom. Rev Inst Med Trop Sao Paulo 53:213-7
Suntravat, Montamas; Yusuksawad, Mariem; Sereemaspun, Amornpun et al. (2011) Effect of purified Russell's viper venom-factor X activator (RVV-X) on renal hemodynamics, renal functions, and coagulopathy in rats. Toxicon 58:230-8

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