: Over the next five years the NIGMS P41 Proteomics Research Center for Integrative Biology Technology Research and Development (TR&D) efforts will focus on the needs of the biomedical research community in the context of Driving Biomedical Projects (DBPs) and collaborative projects that challenge the Center's technical capabilities. The three tightly integrated TR&Ds emphasize approaches for broadly characterizing proteins that include analysis of specific functions and activities, post-translational modifications, and other proteoforms;instrumental developments that enable more sensitive, quantitative, and high throughput proteomic measurements, including analyses of very small samples;and development of computational capabilities that provide improved quantitative proteomics measurements and facilitate proteomics data interpretation and subsequent integration with other data types, as well as their dissemination. The Center's technology developments will be evaluated in the context of a geographically diverse set of DBPs selected on the basis of their scientific and biomedical relevance, as well as their capacity to benefit from the Center's capabilities. These projects are essential to the Center, providing both direction for the early stage TR&D activities and test-beds for the developing technologies. The scope of the new DBP applications include the areas of protein quantification in signal transduction pathways, lipid-binding protein receptors, proteomic analysis of single pancreatic islets in diabetes, neuroproteomics, microbiome impact on lung transplant stability, oncogene regulation by PTMs, characterization of the intestinal host-pathogen interactome, embryonic survival and implantation, and functional annotation of drug resistant Mtb strains. In addition to these DBPs will be a host of new collaborative and service projects that benefit from distinct Center capabilities. The Center will train researchers in the ue of the adva

Public Health Relevance

Advances in instrumentation, experimental approaches, and methods for improving both data quality and integration, will lead to sensitive, quantitative measurements ofthe true proteome. Molecular insights on the nature and operation of cells, signaling pathways/networks, and other processes relevant to human health and disease state progression will be revealed. The Center serves the biomedical community by developing, integrating, and disseminating proteomic technologies for important biological applications.

National Institute of Health (NIH)
National Institute of General Medical Sciences (NIGMS)
Biotechnology Resource Grants (P41)
Project #
Application #
Study Section
Special Emphasis Panel (ZRG1-BST-N (40))
Program Officer
Sheeley, Douglas
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Battelle Pacific Northwest Laboratories
United States
Zip Code
Zheng, Xueyun; Dupuis, Kevin T; Aly, Noor A et al. (2018) Utilizing ion mobility spectrometry and mass spectrometry for the analysis of polycyclic aromatic hydrocarbons, polychlorinated biphenyls, polybrominated diphenyl ethers and their metabolites. Anal Chim Acta 1037:265-273
Zhu, Ying; Clair, Geremy; Chrisler, William B et al. (2018) Proteomic Analysis of Single Mammalian Cells Enabled by Microfluidic Nanodroplet Sample Preparation and Ultrasensitive NanoLC-MS. Angew Chem Int Ed Engl 57:12370-12374
MacLean, Brendan X; Pratt, Brian S; Egertson, Jarrett D et al. (2018) Using Skyline to Analyze Data-Containing Liquid Chromatography, Ion Mobility Spectrometry, and Mass Spectrometry Dimensions. J Am Soc Mass Spectrom 29:2182-2188
Liu, Tao; Rodland, Karin D; Smith, Richard D (2018) Characterization of the Ovarian Tumor Peptidome. Vitam Horm 107:515-531
Schwartz, Janice B; Gallagher, J Christopher; Jorde, Rolf et al. (2018) Determination of Free 25(OH)D Concentrations and Their Relationships to Total 25(OH)D in Multiple Clinical Populations. J Clin Endocrinol Metab 103:3278-3288
Nagy, Gabe; Attah, Isaac K; Garimella, Sandilya V B et al. (2018) Unraveling the isomeric heterogeneity of glycans: ion mobility separations in structures for lossless ion manipulations. Chem Commun (Camb) 54:11701-11704
Stanfill, Bryan A; Nakayasu, Ernesto S; Bramer, Lisa M et al. (2018) Quality Control Analysis in Real-time (QC-ART): A Tool for Real-time Quality Control Assessment of Mass Spectrometry-based Proteomics Data. Mol Cell Proteomics 17:1824-1836
Ortega, Corrie; Frando, Andrew; Webb-Robertson, Bobbie-Jo et al. (2018) A Global Survey of ATPase Activity in Plasmodium falciparum Asexual Blood Stages and Gametocytes. Mol Cell Proteomics 17:111-120
Orton, Daniel J; Tfaily, Malak M; Moore, Ronald J et al. (2018) A Customizable Flow Injection System for Automated, High Throughput, and Time Sensitive Ion Mobility Spectrometry and Mass Spectrometry Measurements. Anal Chem 90:737-744
Shi, Tujin; Gaffrey, Matthew J; Fillmore, Thomas L et al. (2018) Facile carrier-assisted targeted mass spectrometric approach for proteomic analysis of low numbers of mammalian cells. Commun Biol 1:103

Showing the most recent 10 out of 238 publications