Abstract

Since its inception the Yeast Resource Center has specialized in extracting information about protein function from genome sequence. We began when the first eukaryotic genome was completed (budding yeast) and developed an array of technologies to decipher protein function from genome sequence. In this application, we make the natural progression towards understanding how protein variation affects protein levels, modification, function and structure. We propose to develop technologies in four areas: 1) To identify different protein variants or isoforms. 2) To quantify proteins by mass spectrometry with a focus on determining absolute levels of a protein. 3) To study how protein variation affects protein function. 4) To determine the structures of protein complexes not amenable to high-resolution methods such as x-ray crystallography and NMR.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Biotechnology Resource Grants (P41)
Project #
3P41GM103533-20S1
Application #
9319391
Study Section
Special Emphasis Panel (ZRG1)
Program Officer
Sheeley, Douglas
Project Start
Project End
Budget Start
2015-07-01
Budget End
2017-06-30
Support Year
20
Fiscal Year
2016
Total Cost
Indirect Cost

  Institution

Name
University of Washington
Department
Type
DUNS #
605799469
City
Seattle
State
WA
Country
United States
Zip Code
98195

  Publications

Smukowski Heil, Caiti; Burton, Joshua N; Liachko, Ivan et al. (2018) Identification of a novel interspecific hybrid yeast from a metagenomic spontaneously inoculated beer sample using Hi-C. Yeast 35:71-84
Ziegler, Yvonne S; Moresco, James J; Tu, Patricia G et al. (2018) Proteomic analysis identifies highly expressed plasma membrane proteins for detection and therapeutic targeting of specific breast cancer subtypes. Clin Proteomics 15:30
Nishimura, Noriyuki; Tsuchiya, Wataru; Moresco, James J et al. (2018) Control of seed dormancy and germination by DOG1-AHG1 PP2C phosphatase complex via binding to heme. Nat Commun 9:2132
McClatchy, Daniel B; Ma, Yuanhui; Liem, David A et al. (2018) Quantitative temporal analysis of protein dynamics in cardiac remodeling. J Mol Cell Cardiol 121:163-172
Starita, Lea M; Islam, Muhtadi M; Banerjee, Tapahsama et al. (2018) A Multiplex Homology-Directed DNA Repair Assay Reveals the Impact of More Than 1,000 BRCA1 Missense Substitution Variants on Protein Function. Am J Hum Genet 103:498-508
Wang, Zheng; Wu, Catherine; Aslanian, Aaron et al. (2018) Defective RNA polymerase III is negatively regulated by the SUMO-Ubiquitin-Cdc48 pathway. Elife 7:
Andeen, Nicole K; Yang, Han-Yin; Dai, Dao-Fu et al. (2018) DnaJ Homolog Subfamily B Member 9 Is a Putative Autoantigen in Fibrillary GN. J Am Soc Nephrol 29:231-239
Helgeson, Luke A; Zelter, Alex; Riffle, Michael et al. (2018) Human Ska complex and Ndc80 complex interact to form a load-bearing assembly that strengthens kinetochore-microtubule attachments. Proc Natl Acad Sci U S A 115:2740-2745
Fong, Kimberly K; Zelter, Alex; Graczyk, Beth et al. (2018) Novel phosphorylation states of the yeast spindle pole body. Biol Open 7:
González, Delfina P; Lamb, Helen V; Partida, Diana et al. (2018) CBD-1 organizes two independent complexes required for eggshell vitelline layer formation and egg activation in C. elegans. Dev Biol 442:288-300

Showing the most recent 10 out of 372 publications