Abstract

The Center will provide training, support, and guidance for biomedical researchers interested in the field of quantitative proteomics. Researchers from across the nation will come to the Center to acquire diverse and valuable proteomics knowledge and skills that can then be implemented at their own institutions. NCQBCS staff will guide visitors through the development of experimental strategies, the preparation of biological samples, the implementation of liquid chromatography tandem mass spectrometry (LC-MS/MS) instrumentation and methods, and the utilization of informatics tools for the analysis of proteomics data. These distinct areas of training, when combined, will enable the complete quantitative analysis of proteomic systems. The majority of NCQBCS trainees will receive hands-on, one-on-one instruction in a dedicated state-of-the-art 600 square foot NCQBCS Training Institute.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Biotechnology Resource Grants (P41)
Project #
5P41GM108538-05
Application #
9988436
Study Section
Special Emphasis Panel (ZRG1)
Project Start
Project End
Budget Start
2020-07-01
Budget End
2021-06-30
Support Year
5
Fiscal Year
2020
Total Cost
Indirect Cost

  Institution

Name
University of Wisconsin Madison
Department
Type
DUNS #
161202122
City
Madison
State
WI
Country
United States
Zip Code
53715

  Publications

Hutchins, Paul D; Russell, Jason D; Coon, Joshua J (2018) LipiDex: An Integrated Software Package for High-Confidence Lipid Identification. Cell Syst 6:621-625.e5
Jha, Pooja; McDevitt, Molly T; Gupta, Rahul et al. (2018) Systems Analyses Reveal Physiological Roles and Genetic Regulators of Liver Lipid Species. Cell Syst 6:722-733.e6
Hebert, Alexander S; Prasad, Satendra; Belford, Michael W et al. (2018) Comprehensive Single-Shot Proteomics with FAIMS on a Hybrid Orbitrap Mass Spectrometer. Anal Chem 90:9529-9537
Mitok, Kelly A; Freiberger, Elyse C; Schueler, Kathryn L et al. (2018) Islet proteomics reveals genetic variation in dopamine production resulting in altered insulin secretion. J Biol Chem 293:5860-5877
Lee, Ji-Hoon; Mand, Michael R; Kao, Chung-Hsuan et al. (2018) ATM directs DNA damage responses and proteostasis via genetically separable pathways. Sci Signal 11:
Rhoads, Timothy W; Burhans, Maggie S; Chen, Vincent B et al. (2018) Caloric Restriction Engages Hepatic RNA Processing Mechanisms in Rhesus Monkeys. Cell Metab 27:677-688.e5
Chen, Zhengwei; Yu, Qing; Hao, Ling et al. (2018) Site-specific characterization and quantitation of N-glycopeptides in PKM2 knockout breast cancer cells using DiLeu isobaric tags enabled by electron-transfer/higher-energy collision dissociation (EThcD). Analyst 143:2508-2519
Jiang, Xiaoyue; Xiang, Feng; Jia, Chenxi et al. (2018) Relative Quantitation of Neuropeptides at Multiple Developmental Stages of the American Lobster Using N, N-Dimethyl Leucine Isobaric Tandem Mass Tags. ACS Chem Neurosci 9:2054-2063
Lapointe, Christopher P; Stefely, Jonathan A; Jochem, Adam et al. (2018) Multi-omics Reveal Specific Targets of the RNA-Binding Protein Puf3p and Its Orchestration of Mitochondrial Biogenesis. Cell Syst 6:125-135.e6
Overmyer, Katherine A; Tyanova, Stefka; Hebert, Alex S et al. (2018) Multiplexed proteome analysis with neutron-encoded stable isotope labeling in cells and mice. Nat Protoc 13:293-306

Showing the most recent 10 out of 32 publications