This proposal describes the need for and outline of a Biomedical Technology Research Resource entitled Center on Macromolecular Dynamics by NMR Spectroscopy (CoMD/NMR) and situated at the New York Structural Biology Center. The focus of CoMD/NMR is technical development and application of NMR spin relaxation and associated methods for characterizing protein and nucleic acid conformational dynamics in biological processes including ligand recognition, allosterism, catalysis, and folding. The central challenge addressed by CoMD/NMR is to break down the high activation barrier to using advanced NMR spectroscopic and computational methods and thus to make available the benefits of these sophisticated approaches to the wider biological research community. CoMD/NMR is the platform for meeting this challenge through Technological Research and Development (TR&D) Projects, Driving Biomedical Projects (DBPs), Collaboration and Service, Training, and Dissemination components of the Resource. The TR&D component of CoMD/NMR will develop new and extended methods for characterizing and interpreting macromolecular dynamics, with a strong focus on expanding the general ranges of time scales and masses of targets accessible for study. The dual needs for integrating experimental, computational, and theoretical methods (within the TR&D component) and for interfacing with key biological researchers (within the DBP and Collaboration components) make CoMD/NMR essential. In particular, CoMD/NMR addresses four primary obstacles: (i) future technology developments requires combined advances in sample preparation, NMR hardware and methods development, and integration with computer methods; (ii) access to NMR spectrometers at multiple static magnetic fields, as well as specialty equipment such as dynamic nuclear polarization and field cycling probes, is key to many emerging methods; (iii) transfer of technology to the general biological community has been hindered by the complexity of the methods, absence of integrated pipelines to facilitate data acquisition and analysis by non- specialists, and lack of infrastructure in individual laboratories to translate these methods for general use; and (iv) biologists with research programs that would benefit from these methods are unaware of this potential and lack expertise to incorporate these approaches into their own research. Technology development proceeds most effectively when driven by challenging applications; accordingly, CoMD/NMR will work closely with outstanding local and national investigators in DBPs designed to motivate and test emerging technology. The resources of CoMD/NMR will be broadly available to the biomedical research community through collaboration and service activities together with expansive training and dissemination programs. The basic research developments of CoMD/NMR will impact a diverse range of biological research with human health relatedness, including degenerative diseases, metabolic disorders, and cancer.

Public Health Relevance

This proposal describes the need for and outline of a Center on Macromolecular Dynamics by NMR Spectroscopy (CoMD/NMR) situated at the New York Structural Biology Center. The focus of CoMD/NMR is technical development and application of NMR spin relaxation and associated methods for characterizing protein and nucleic acid conformational dynamics in biological processes including ligand recognition, allosterism, catalysis, and folding. The basic research developments of this proposal will impact a diverse range of biological research with human health relatedness, including degenerative diseases, metabolic disorders, and cancer.

Agency
National Institute of Health (NIH)
Institute
National Institute of General Medical Sciences (NIGMS)
Type
Biotechnology Resource Grants (P41)
Project #
5P41GM118302-04
Application #
9967018
Study Section
Special Emphasis Panel (ZRG1)
Program Officer
Krepkiy, Dmitriy
Project Start
2017-06-01
Project End
2022-05-31
Budget Start
2020-06-01
Budget End
2021-05-31
Support Year
4
Fiscal Year
2020
Total Cost
Indirect Cost
Name
New York Structural Biology Center
Department
Type
DUNS #
011191520
City
New York
State
NY
Country
United States
Zip Code
10027
Hsu, Andrew; O'Brien, Paul A; Bhattacharya, Shibani et al. (2018) Enhanced spectral density mapping through combined multiple-field deuterium 13CH2D methyl spin relaxation NMR spectroscopy. Methods 138-139:76-84
Koss, Hans; Rance, Mark; Palmer 3rd, Arthur G (2018) General Expressions for Carr-Purcell-Meiboom-Gill Relaxation Dispersion for N-Site Chemical Exchange. Biochemistry 57:4753-4763
Hsu, Andrew; Ferrage, Fabien; Palmer 3rd, Arthur G (2018) Analysis of NMR Spin-Relaxation Data Using an Inverse Gaussian Distribution Function. Biophys J 115:2301-2309
Vugmeyster, Liliya; Griffin, Aaron; Ostrovsky, Dmitry et al. (2018) Correlated motions of C'-N and C?-C? pairs in protonated and per-deuterated GB3. J Biomol NMR 72:39-54
O'Brien, Paul A; Palmer 3rd, Arthur G (2018) TROSY pulse sequence for simultaneous measurement of the 15N R1 and {1H}-15N NOE in deuterated proteins. J Biomol NMR 70:205-209
Fritzsching, Keith J; Itin, Boris; McDermott, Ann E (2018) N,N-Diethylmethylamine as lineshape standard for NMR above 130?K. J Magn Reson 287:110-112
Rogawski, Rivkah; Sergeyev, Ivan V; Zhang, Yinglu et al. (2017) NMR Signal Quenching from Bound Biradical Affinity Reagents in DNP Samples. J Phys Chem B 121:10770-10781
Rogawski, Rivkah; McDermott, Ann E (2017) New NMR tools for protein structure and function: Spin tags for dynamic nuclear polarization solid state NMR. Arch Biochem Biophys 628:102-113