Folate cofactors are essential for the growth of all organisms. While man and animals are able to take folates from foods, most microorganisms have to synthesize folates de novo. Thus, enzymes involved in folate biosynthesis are targets of antimicrobial agents. 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase (HPPK) catalyzes the first reaction in the folate biosynthesis pathway. The long-term goals of the project is to elucidate the structure and mechanism of HPPK and to design inhibitors for the enzyme. HPPK was expressed in E. coli at high level. MALDI-MS analysis was used to determine the molecular weight of the recombinant HPPK. The result showed that the N-terminal methionine was removed in vivo.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
3P41RR000480-28S1
Application #
6258874
Study Section
Project Start
1997-06-01
Project End
1999-11-30
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
28
Fiscal Year
1999
Total Cost
Indirect Cost
Name
Michigan State University
Department
Type
DUNS #
193247145
City
East Lansing
State
MI
Country
United States
Zip Code
48824
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Lopez-Lara, I M; Orgambide, G; Dazzo, F B et al. (1993) Characterization and symbiotic importance of acidic extracellular polysaccharides of Rhizobium sp. strain GRH2 isolated from acacia nodules. J Bacteriol 175:2826-32
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