The system we are developing to help automate our HVEM has four basic components: 1) An automation control computer (an IBM PC running DOS); 2) The micro-electronic components that are themselves directly controlled by the IBM PC; 3) The electronics that connect the micro-electronic components to the HVEM; and 4) A microscope control computer (an Apple, Macintosh running MacOS) that will provide a convenient interface between the user and both the above system and the digital camera, soon be installed on our HVEM. Micro-electronic components have been assembled with the automation control computer. Low level programming that allows the micro electronics to be controlled via a standard computer serial interface has been completed. The interconnecting electronics for all the control tasks needed for automated tomography should be completed in the same time frame as the installation of the Gatan slow scan CCD camera. These controls include specimen stage translation and tilt, focus, and limited beam tilt. Additional controls for magnification change and beam brightness will be added once the first phase is completed. Our plan for the microscope control computer is at present to use the same Apple Macintosh computer that will host the Gatan camera. Programs for viewing, compressing, and decompressing image files have been written. These programs will be enhanced once the Gatan camera has been delivered and the performance of the programs can be tested. A simple network server that allows for remote control of the microscope and the Gatan camera is being written. This server will allow other computers, such as our SGI UNIX workstations, to help with image processing tasks and will permit remote client programs to use the microscope and camera. A framework client program has been written in Java to allow for testing the remote microscopy controls. ?

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR000592-28
Application #
6278673
Study Section
Project Start
1997-12-01
Project End
1998-11-30
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
28
Fiscal Year
1998
Total Cost
Indirect Cost
Name
University of Colorado at Boulder
Department
Type
DUNS #
City
Boulder
State
CO
Country
United States
Zip Code
80309
Giddings Jr, Thomas H; Morphew, Mary K; McIntosh, J Richard (2017) Preparing Fission Yeast for Electron Microscopy. Cold Spring Harb Protoc 2017:
Zhao, Xiaowei; Schwartz, Cindi L; Pierson, Jason et al. (2017) Three-Dimensional Structure of the Ultraoligotrophic Marine Bacterium ""Candidatus Pelagibacter ubique"". Appl Environ Microbiol 83:
Brown, Joanna R; Schwartz, Cindi L; Heumann, John M et al. (2016) A detailed look at the cytoskeletal architecture of the Giardia lamblia ventral disc. J Struct Biol 194:38-48
Saheki, Yasunori; Bian, Xin; Schauder, Curtis M et al. (2016) Control of plasma membrane lipid homeostasis by the extended synaptotagmins. Nat Cell Biol 18:504-15
Höög, Johanna L; Lacomble, Sylvain; Bouchet-Marquis, Cedric et al. (2016) 3D Architecture of the Trypanosoma brucei Flagella Connector, a Mobile Transmembrane Junction. PLoS Negl Trop Dis 10:e0004312
Park, J Genevieve; Palmer, Amy E (2015) Properties and use of genetically encoded FRET sensors for cytosolic and organellar Ca2+ measurements. Cold Spring Harb Protoc 2015:pdb.top066043
McCoy, Kelsey M; Tubman, Emily S; Claas, Allison et al. (2015) Physical limits on kinesin-5-mediated chromosome congression in the smallest mitotic spindles. Mol Biol Cell 26:3999-4014
Höög, Johanna L; Lötvall, Jan (2015) Diversity of extracellular vesicles in human ejaculates revealed by cryo-electron microscopy. J Extracell Vesicles 4:28680
Marc, Robert E; Anderson, James R; Jones, Bryan W et al. (2014) The AII amacrine cell connectome: a dense network hub. Front Neural Circuits 8:104
Weber, Britta; Tranfield, Erin M; Höög, Johanna L et al. (2014) Automated stitching of microtubule centerlines across serial electron tomograms. PLoS One 9:e113222

Showing the most recent 10 out of 84 publications