This year our facility hosted three visitors, each of whom spent one week with us to learn various methods of specimen preparation and computer-assisted image analysis. Two of the visitors, Drs. Karl Ekwall and Trisha Davis, participated in mini-workshops designed to teach specimen preparation, immunolocalization, tomography, and serial reconstruction. These mini-workshops have led to official collaborations with Drs. Ekwall and Davis. In addition, our long-time collaborator, Dr. Mary Porter visited the lab to learn the steps required for image analysis of axonemal dynein in Chlamydomonas. During Dr. Porter~s visit she also met with David Mastronarde and Eileen O'Toole to obtain the programs needed to set up the image analysis protocols in her laboratory in Minnesota. The Boulder Laboratory for 3-D Fine Structure home page (http://bio3d.colorado.edu) has also been updated to contain a web-install option to download the software developed in our lab. The IMOD modeling software continues to be a useful program for our laboratory and to several laboratories around the world. Several improvements to the IMOD program have been implemented by David Mastronarde further to improve its usefulness
Giddings Jr, Thomas H; Morphew, Mary K; McIntosh, J Richard (2017) Preparing Fission Yeast for Electron Microscopy. Cold Spring Harb Protoc 2017: |
Zhao, Xiaowei; Schwartz, Cindi L; Pierson, Jason et al. (2017) Three-Dimensional Structure of the Ultraoligotrophic Marine Bacterium ""Candidatus Pelagibacter ubique"". Appl Environ Microbiol 83: |
Brown, Joanna R; Schwartz, Cindi L; Heumann, John M et al. (2016) A detailed look at the cytoskeletal architecture of the Giardia lamblia ventral disc. J Struct Biol 194:38-48 |
Saheki, Yasunori; Bian, Xin; Schauder, Curtis M et al. (2016) Control of plasma membrane lipid homeostasis by the extended synaptotagmins. Nat Cell Biol 18:504-15 |
Höög, Johanna L; Lacomble, Sylvain; Bouchet-Marquis, Cedric et al. (2016) 3D Architecture of the Trypanosoma brucei Flagella Connector, a Mobile Transmembrane Junction. PLoS Negl Trop Dis 10:e0004312 |
Park, J Genevieve; Palmer, Amy E (2015) Properties and use of genetically encoded FRET sensors for cytosolic and organellar Ca2+ measurements. Cold Spring Harb Protoc 2015:pdb.top066043 |
McCoy, Kelsey M; Tubman, Emily S; Claas, Allison et al. (2015) Physical limits on kinesin-5-mediated chromosome congression in the smallest mitotic spindles. Mol Biol Cell 26:3999-4014 |
Höög, Johanna L; Lötvall, Jan (2015) Diversity of extracellular vesicles in human ejaculates revealed by cryo-electron microscopy. J Extracell Vesicles 4:28680 |
Marc, Robert E; Anderson, James R; Jones, Bryan W et al. (2014) The AII amacrine cell connectome: a dense network hub. Front Neural Circuits 8:104 |
Weber, Britta; Tranfield, Erin M; Höög, Johanna L et al. (2014) Automated stitching of microtubule centerlines across serial electron tomograms. PLoS One 9:e113222 |
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