A total of 236 hours of instrument time has been spent in a combination of routine maintenance, improvements, and repairs of the HVEM and its components. Routine maintenance included replacing apertures, correcting astigmatism and beam tilt, changing the filament, replacing vacuum gauges, and doing magnification calibrations. Additional planned maintenance included rebuilding the camera vacuum isolation valve and replacing air pressure components. Maintenance that did not affect microscope availability included rebuilding one of our 1MeV linear acceleration tubes. Total time spent on maintenance was 112 hours. Improvements to the microscope included adding electronics for microscope automation and getting ready for installation of the Gatan slow-scan CCD camera. Additional non-microscope time was spent wiring and programming the microscope automation system that is currently being installed. Total microscope time spent on improvements was 80 hours. Most of the downtime occurred during a week that Kremer was on vacation and was caused by a faulty air pressure regulator and a jammed objective aperture. Total downtime was 44 hours. The percent usage reflects the percent of the days that the microscope is being used. Values for 1997 are a year-to-date percentage. HVEM downtime shows the number of hours the microscope wasn_t available for imaging because of planned maintenance and improvements or because of unplanned repairs. The values for 1997 are projected values and include time for installation of the automation computer and new imaging camera.
| Giddings Jr, Thomas H; Morphew, Mary K; McIntosh, J Richard (2017) Preparing Fission Yeast for Electron Microscopy. Cold Spring Harb Protoc 2017: |
| Zhao, Xiaowei; Schwartz, Cindi L; Pierson, Jason et al. (2017) Three-Dimensional Structure of the Ultraoligotrophic Marine Bacterium ""Candidatus Pelagibacter ubique"". Appl Environ Microbiol 83: |
| Brown, Joanna R; Schwartz, Cindi L; Heumann, John M et al. (2016) A detailed look at the cytoskeletal architecture of the Giardia lamblia ventral disc. J Struct Biol 194:38-48 |
| Saheki, Yasunori; Bian, Xin; Schauder, Curtis M et al. (2016) Control of plasma membrane lipid homeostasis by the extended synaptotagmins. Nat Cell Biol 18:504-15 |
| Höög, Johanna L; Lacomble, Sylvain; Bouchet-Marquis, Cedric et al. (2016) 3D Architecture of the Trypanosoma brucei Flagella Connector, a Mobile Transmembrane Junction. PLoS Negl Trop Dis 10:e0004312 |
| Höög, Johanna L; Lötvall, Jan (2015) Diversity of extracellular vesicles in human ejaculates revealed by cryo-electron microscopy. J Extracell Vesicles 4:28680 |
| Park, J Genevieve; Palmer, Amy E (2015) Properties and use of genetically encoded FRET sensors for cytosolic and organellar Ca2+ measurements. Cold Spring Harb Protoc 2015:pdb.top066043 |
| McCoy, Kelsey M; Tubman, Emily S; Claas, Allison et al. (2015) Physical limits on kinesin-5-mediated chromosome congression in the smallest mitotic spindles. Mol Biol Cell 26:3999-4014 |
| Marc, Robert E; Anderson, James R; Jones, Bryan W et al. (2014) The AII amacrine cell connectome: a dense network hub. Front Neural Circuits 8:104 |
| Weber, Britta; Tranfield, Erin M; Höög, Johanna L et al. (2014) Automated stitching of microtubule centerlines across serial electron tomograms. PLoS One 9:e113222 |
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