Abstract

In a preparation of linker chain L1 and 24 kD, an impurity was recently identified as a possible additional linker chain L4. The amino acid sequence of L4 was determined. The molecular mass of L4 was measured by matrix-assisted laser desorption mass spectrometry. A small, but significant deviation between calculated and measured mass of L4 found and is currently being investigated. The sugar portion of linker chains of earthworm hemoglobins is possibly involved in the assemblage of the macromolecular structure of the intact molecule. The glycosylation of the different linker chains of earthworm hemoglobin is still controversial. Matrix-assisted laser desorption mass spectrometry is being used to monitor the deglycosylation of earthworm hemoglobin linker chains. Molecular weight measurements of linker chains L1 and L2 revealed that the enzymatic deglycosylation by N-glycanase was incomplete.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR000862-23
Application #
5221613
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
23
Fiscal Year
1996
Total Cost
Indirect Cost

  Publications

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Krutchinsky, Andrew N; Padovan, Júlio C; Cohen, Herbert et al. (2015) Optimizing electrospray interfaces using slowly diverging conical duct (ConDuct) electrodes. J Am Soc Mass Spectrom 26:659-67
Oricchio, Elisa; Papapetrou, Eirini P; Lafaille, Fabien et al. (2014) A cell engineering strategy to enhance the safety of stem cell therapies. Cell Rep 8:1677-1685
Zhong, Yu; Morris, Deanna H; Jin, Lin et al. (2014) Nrbf2 protein suppresses autophagy by modulating Atg14L protein-containing Beclin 1-Vps34 complex architecture and reducing intracellular phosphatidylinositol-3 phosphate levels. J Biol Chem 289:26021-37
Xue, John Z; Woo, Eileen M; Postow, Lisa et al. (2013) Chromatin-bound Xenopus Dppa2 shapes the nucleus by locally inhibiting microtubule assembly. Dev Cell 27:47-59
Indiani, Chiara; O'Donnell, Mike (2013) A proposal: Source of single strand DNA that elicits the SOS response. Front Biosci (Landmark Ed) 18:312-23

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