We are making a major effort to develop the utility of mass spectrometry to assist in the definition of integral membrane proteins and (especially ion channels) at atomic resolutions using diffraction - methods. Currently, the structure effort consists of three components: (a) work on high-level expression and purification of ion channels, (b) development of methods for growing two dimensional crystals (for electron microscopy studies) using small quantities of channel protein and (c) X-ray crystallographic studies of ion channel domains and soluble regulatory proteins that bind to ion channels. Our goal is to obtain high-resolution structures of the full, integral membrane ion channel through X-ray crystallography. By combining the exquisitely detailed functional information obtainable using patchrecording methods with three-dimensional structural data from X-ray crystallography, the central questions concerning the mechanisms of ion channel function can be answered. Mass spectrometry is beginning to have a major impact in facilitating this work - e.g., in elucidating compact domains of the ion channels.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR000862-27
Application #
6307594
Study Section
Project Start
1999-12-01
Project End
2000-11-30
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
27
Fiscal Year
2000
Total Cost
$8,199
Indirect Cost
Name
Rockefeller University
Department
Type
DUNS #
071037113
City
New York
State
NY
Country
United States
Zip Code
10065
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