This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.The Allis laboratory at The Rockefeller University studies how post-translational modifications on histones affect the biology of chromatin. In this regard, we are working towards a complete map of post-translational modifications on histone H3 from Tetrahymena thermophila. Dr. Taverna has isolated H3, and used column chromatography to resolve the H3 into 20 fractions that each contains differential modifications. We are using a combination of single and tandem mass spectrometry to identify the sites of and types of modifications. Current efforts on this project: 1) finish mapping the post-translational modifications, and 2) determine the relative abundances of these modifications at each amino acid position through stable isotope incorporation experiments in combination with mass spectrometry. Over the past several months, electron transfer decay is being used to elucidate the complex arrays of modifications that are obseved on histone H3.
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