This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.In order to gain understanding on the assembly and maintenance of the nuclear pore complex it is of interest to determine the turnover of its components. Using heavy-isotope metabolic labeling, immunoisolation of the Nup107 protein complex after increasing lengths of time (0, 3, 6, 10, 15, 22, and 32 hours)in cell culture, and mass spectrometry, we followed the half-lives of Nup107 and associated proteins.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR000862-35
Application #
7722279
Study Section
Special Emphasis Panel (ZRG1-BCMB-Q (40))
Project Start
2008-03-01
Project End
2009-02-28
Budget Start
2008-03-01
Budget End
2009-02-28
Support Year
35
Fiscal Year
2008
Total Cost
$5,537
Indirect Cost
Name
Rockefeller University
Department
Miscellaneous
Type
Other Domestic Higher Education
DUNS #
071037113
City
New York
State
NY
Country
United States
Zip Code
10065
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