It is proposed to continue to operate and extend the capabilities of the National Resource for the Mass Spectrometric Analysis of Biological Macromolecules. Emphasis will be placed upon the mass spectrometry (MS) of peptides &proteins, for developing proteomic tools for dissecting cellular function, and for developing novel means for studying viral host interactions and the molecular organization of the mammalian brain. The major subdivisions of the Resource activity will be: basic research in MS, collaborative research on challenging biological problems, high-level bioanalytical service, and dissemination of newly developed technologies to the biomedical community. Basic research will involve the development of (1) novel instrumentation for rapid, ultrasensitive tandem MS of peptides &ultra sensitive detection &characterization of phosphopeptides &phosphoproteins, (2) new methods for inducing efficient fragmentation of peptides &small proteins, (3) second generation methods for MS quantitation of proteins, (4) improved methods for studying dynamic protein interactions, (5) improved methods for elucidating the domains structures of proteins, and (6) as test beds for our new technologies, four large-scale applied projects: (i) a comprehensive description of chromosomes, (ii) comprehensive analysis of cellular membranes (iii) study of viral host interactions, and (iv) unraveling the molecular synaptic code in the mammalian brain. Collaborative research will involve definition and study of protein machines ℂcell cycle control;cell division dynamics;control of cellular architecture;DNA replication;control of stress in plants;cellular membrane biogenesis;cell-specific molecular dissection of mammalian synapses;signaling in the brain;mechanism of action of neurotransmitters, therapeutic agents &drugs of abuse in the brain;molecular intervention in Alzheimer's disease;autophagy in axonal dystrophy °eneration;neuropathogenesis of Parkinson's disease;opioid receptor systems in addiction &analgesic response;gene expression &transcription;chromatin biology &epigenetics;telomere organization;analysis of cellular pathways modulated by herpes viruses;molecular analysis of Sindbis alphavirus, hepatitis C, &BVDV infection in host cells, Host interactions of gene products from HIV-1;structure &function of ions channels, comprehensive definition of cellular membranes;lymphocyte differentiation &maintenance;and immunoglobulin-class switching.

National Institute of Health (NIH)
National Center for Research Resources (NCRR)
Biotechnology Resource Grants (P41)
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Special Emphasis Panel (ZRG1-BCMB-Q (40))
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Sheeley, Douglas
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Rockefeller University
Other Domestic Higher Education
New York
United States
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Manning, Lois R; Popowicz, Anthony M; Padovan, Julio C et al. (2017) Gel filtration of dilute human embryonic hemoglobins reveals basis for their increased oxygen binding. Anal Biochem 519:38-41
Boice, Michael; Salloum, Darin; Mourcin, Frederic et al. (2016) Loss of the HVEM Tumor Suppressor in Lymphoma and Restoration by Modified CAR-T Cells. Cell 167:405-418.e13
Chait, Brian T; Cadene, Martine; Olinares, Paul Dominic et al. (2016) Revealing Higher Order Protein Structure Using Mass Spectrometry. J Am Soc Mass Spectrom 27:952-65
Krutchinsky, Andrew N; Padovan, Júlio C; Cohen, Herbert et al. (2015) Maximizing ion transmission from atmospheric pressure into the vacuum of mass spectrometers with a novel electrospray interface. J Am Soc Mass Spectrom 26:649-58
Mast, Fred D; Rachubinski, Richard A; Aitchison, John D (2015) Signaling dynamics and peroxisomes. Curr Opin Cell Biol 35:131-6
Krutchinsky, Andrew N; Padovan, Júlio C; Cohen, Herbert et al. (2015) Optimizing electrospray interfaces using slowly diverging conical duct (ConDuct) electrodes. J Am Soc Mass Spectrom 26:659-67
Oricchio, Elisa; Papapetrou, Eirini P; Lafaille, Fabien et al. (2014) A cell engineering strategy to enhance the safety of stem cell therapies. Cell Rep 8:1677-1685
Zhong, Yu; Morris, Deanna H; Jin, Lin et al. (2014) Nrbf2 protein suppresses autophagy by modulating Atg14L protein-containing Beclin 1-Vps34 complex architecture and reducing intracellular phosphatidylinositol-3 phosphate levels. J Biol Chem 289:26021-37
Xue, John Z; Woo, Eileen M; Postow, Lisa et al. (2013) Chromatin-bound Xenopus Dppa2 shapes the nucleus by locally inhibiting microtubule assembly. Dev Cell 27:47-59
Indiani, Chiara; O'Donnell, Mike (2013) A proposal: Source of single strand DNA that elicits the SOS response. Front Biosci (Landmark Ed) 18:312-23

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