This research application of the Washington University Mass Spectrometry Resource plans: 1. To continue to provide wide range low resolution GCMS service to local, regional, national, and international biomedical investigators who request this assistance. 2. To continue to provide expert technical assistance, analytical services, and interpretive advice on high precision GCMS selected ion monitoring quantitation (SIM-GCMS) of substrate content and stable isotope enrichment in physiologic samples. 3. To develop a fully automated isotope ratio mass spectrometer (IRMS) to permit rapid, high sample volume operation for measurement of stable isotope enrichment in expired and excreted end products of endogenous substrate metabolism. 4. To develop an isotope ratio monitoring GCMS (IRM-GCMS) for high precision quantitative stable isotope analysis with smaller sample size and less sample preparation than with IRMS and with the ability to measure greater tracer dilution than possible with SIM-GCMS. 5. To establish a high resolution mass spectrometry-fast atom ionization facility for the analysis of high molecular weight or thermally labile molecules. 6. To make the newly developed facilities available for use by qualified investigators at the local, regional, national, and international levels.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR000954-09
Application #
3103708
Study Section
(SSS)
Project Start
1975-06-01
Project End
1986-02-28
Budget Start
1985-03-01
Budget End
1986-02-28
Support Year
9
Fiscal Year
1985
Total Cost
Indirect Cost
Name
Washington University
Department
Type
Schools of Medicine
DUNS #
062761671
City
Saint Louis
State
MO
Country
United States
Zip Code
63130
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