An 85 kDa Group VI phospholipase A2 enzyme, designated iPLA2, that does not require Ca2+ for catalysis has recently been cloned from three rodent species. An homologous 88 kDa enzyme has been cloned from human B-lymphocyte lines that contains a 54 amino acid insert not present in the rodent enzymes, but human cells have not previously been observed to express catalytically active iPLA2 isoforms other than the 88 kDa protein. We have cloned cDNA species that encode two distinct iPLA2 isoforms from human pancreatic islet RNA and a human insulinoma cDNA library. One isoform is an 85 kDa protein (SH-iPLA2) and the other an 88 kDa protein (LH-iPLA2). Transcripts encoding both isoforms are also observed in human promonocytic U937 cells. Recombinant SH-iPLA2 and LH-iPLA2 are both catalytically active in the absence of Ca2+ and inhibited by a bromoenol lactone (BEL) suicide substrate, but LH-iPLA2 is activated by ATP, while SH-iPLA2 is not. The human iPLA2 gene has been fou nd to resi de on chromosome 22 in region q13.1 and to contain sixteen exons represented in the LH-iPLA2 transcript. Exon 8 is not represented in the SH-iPLA2 transcript, indicating that it arises by an exon-skipping mechanism of alternative splicing. The amino acid sequence encoded by exon 8 of the human iPLA2 gene is proline-rich and shares a consensus motif of Px5Px8HHPx12Nx4Q with the proline-rich middle linker domains of the Smad proteins DAF-3 and Smad4. Expression of mRNA species encoding two active iPLA2 isoforms with distinguishable catalytic properties in two different types of human cells demonstrated here may have regulatory or functional implications about the roles of products of the iPLA2 gene in cell biologic processes.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR000954-26
Application #
6665864
Study Section
Project Start
2002-08-01
Project End
2003-07-31
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
26
Fiscal Year
2002
Total Cost
$157,506
Indirect Cost
Name
Washington University
Department
Type
DUNS #
062761671
City
Saint Louis
State
MO
Country
United States
Zip Code
63130
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