The main goal is to develop optical biology techniques for visualizing and understanding how external stimuli, such as heat and UV irradiation, induce varieties of gene expression, and how it is related to malignant transformations in mammalian cells. We are developing a molecular reporter system by constructing plasmids containing different reporter gene GFPs (Green Fluorescence Protein) respectively. Initial studies confirm that GFP can be used as a reporter when subcloned with two different promoters, CMV and SV40. The wild type GFP and several mutants did not exhibit any detectable fluorescence when either grown in bacteria or transfected into CHO cells. However, when GFP mutant S65C was subcloned with the CMV promoter it emitted a strong green fluorescence both in bacteria and CHO cells, i.e. reporting gene expression.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001192-17
Application #
5222600
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
17
Fiscal Year
1996
Total Cost
Indirect Cost
Paugh, Jerry R; Alfonso-Garcia, Alba; Nguyen, Andrew Loc et al. (2018) Characterization of expressed human meibum using hyperspectral stimulated Raman scattering microscopy. Ocul Surf :
Verdel, Nina; Lentsch, Griffin; Balu, Mihaela et al. (2018) Noninvasive assessment of skin structure by combined photothermal radiometry and optical spectroscopy: coregistration with multiphoton microscopy. Appl Opt 57:D117-D122
Friedman, Jacob E; Dobrinskikh, Evgenia; Alfonso-Garcia, Alba et al. (2018) Pyrroloquinoline quinone prevents developmental programming of microbial dysbiosis and macrophage polarization to attenuate liver fibrosis in offspring of obese mice. Hepatol Commun 2:313-328
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Alfonso-García, Alba; Paugh, Jerry; Farid, Marjan et al. (2017) A machine learning framework to analyze hyperspectral stimulated Raman scattering microscopy images of expressed human meibum. J Raman Spectrosc 48:803-812
Takesh, Thair; Sargsyan, Anik; Anbarani, Afarin et al. (2017) Effects of a Novel Whitening Formulation on Dental Enamel. Dentistry (Sunnyvale) 7:
Choi, Bernard; Tan, Wenbin; Jia, Wangcun et al. (2016) The Role of Laser Speckle Imaging in Port-Wine Stain Research: Recent Advances and Opportunities. IEEE J Sel Top Quantum Electron 2016:
Wang, Mingqiu; Ravindranath, Shreyas R; Rahim, Maha K et al. (2016) Evolution of Multivalent Nanoparticle Adhesion via Specific Molecular Interactions. Langmuir 32:13124-13136

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