Abstract

Microscopic examination of hard dental tissue is difficult, especially at high magnifications. Visualization of the z-axis dimension of hard tissue is particularly problematic, especially when quantitative results using non-destructive techniques are required. It was the aim of this investigation to develop a technique for visualizing, documenting and quantifying ablation and surface changes in dental hard tissues as a consequence of laser irradiation or conventional dental drill usage. Confocal laser scanning microscopy (LSM) is a perfect tool for non-invasive thin optical sectioning of reflecting surfaces and 3-D reconstruction. Using confocal LSM, visualization of surface and crater topography can be achieved. Measurement of cavity depth and profiles in different planes of section is also possible, allowing observation of ablative effects in 3 dimensions.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001192-17
Application #
5222611
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
17
Fiscal Year
1996
Total Cost
Indirect Cost

  Publications

Paugh, Jerry R; Alfonso-Garcia, Alba; Nguyen, Andrew Loc et al. (2018) Characterization of expressed human meibum using hyperspectral stimulated Raman scattering microscopy. Ocul Surf :
Verdel, Nina; Lentsch, Griffin; Balu, Mihaela et al. (2018) Noninvasive assessment of skin structure by combined photothermal radiometry and optical spectroscopy: coregistration with multiphoton microscopy. Appl Opt 57:D117-D122
Friedman, Jacob E; Dobrinskikh, Evgenia; Alfonso-Garcia, Alba et al. (2018) Pyrroloquinoline quinone prevents developmental programming of microbial dysbiosis and macrophage polarization to attenuate liver fibrosis in offspring of obese mice. Hepatol Commun 2:313-328
Kennedy, Gordon T; Lentsch, Griffin R; Trieu, Brandon et al. (2017) Solid tissue simulating phantoms having absorption at 970 nm for diffuse optics. J Biomed Opt 22:76013
Takesh, Thair; Sargsyan, Anik; Lee, Matthew et al. (2017) Evaluating the Whitening and Microstructural Effects of a Novel Whitening Strip on Porcelain and Composite Dental Materials. Dentistry (Sunnyvale) 7:
Jonscher, Karen R; Stewart, Michael S; Alfonso-Garcia, Alba et al. (2017) Early PQQ supplementation has persistent long-term protective effects on developmental programming of hepatic lipotoxicity and inflammation in obese mice. FASEB J 31:1434-1448
Alfonso-García, Alba; Paugh, Jerry; Farid, Marjan et al. (2017) A machine learning framework to analyze hyperspectral stimulated Raman scattering microscopy images of expressed human meibum. J Raman Spectrosc 48:803-812
Takesh, Thair; Sargsyan, Anik; Anbarani, Afarin et al. (2017) Effects of a Novel Whitening Formulation on Dental Enamel. Dentistry (Sunnyvale) 7:
Malacrida, Leonel; Astrada, Soledad; Briva, Arturo et al. (2016) Spectral phasor analysis of LAURDAN fluorescence in live A549 lung cells to study the hydration and time evolution of intracellular lamellar body-like structures. Biochim Biophys Acta 1858:2625-2635
Choi, Bernard; Tan, Wenbin; Jia, Wangcun et al. (2016) The Role of Laser Speckle Imaging in Port-Wine Stain Research: Recent Advances and Opportunities. IEEE J Sel Top Quantum Electron 2016:

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