We have been unable to confirm acidification of L1210 leukemia cells at VP-16 concentration of 150nM or less. Our continuation of these experiments fails to show acidification of these cells by VP-16 alone at a concentration of up to 420 nM, which is far in excess of their VP-16 1C50 value of 110nM. The addition of Cyclosporin A to VP-16 also fails to promote cellular acidification which indicates that Cyclosporin enhancement of VP-16 cytotoxicity relates to an alternate mechanism.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001192-17
Application #
5222620
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
17
Fiscal Year
1996
Total Cost
Indirect Cost
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