Abstract

High-grade squamous intraepithelial lesion (H-SIL) is an extremely frequent precancerous cervical lesion, leading to invasive cervical cancer if undetected and untreated. We are currently evaluating two new noninvasive methods for detection and treatment of H-SIL photodynamic therapy (PDT) and frequency domain photon migration (FDPM). PDT induces selective necrosis of dye sensitize malignant tissues when activated with light of appropriate wavelength. FDPM is a near-infrared spectroscopy technique for determining optical properties (i.e. absorption and scattering of light, ua and uUs) of tissues. Our objectives are 1) To determine the feasibility, safety, and efficacy of using PDT to treat H-SIL, 2) To quantitatively determine ua and uUs of normal cervical tissue and H-SIL in vivo, and to calculate physiologic parameters, specifically absolute concentration of oxy/deoxyhemoglobin and water in cervical tissue, 3) To assess the feasibility of differentiating normal cervical tissues from H-SIL based on the optical properties, 4) to monitor how cervical tissue optical and physiological properties change in response to PDT, 5) To optimize PDT light dosimetery and clinical outcome based on optical property measurements.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
2P41RR001192-19
Application #
6280345
Study Section
Project Start
1998-05-15
Project End
1999-03-31
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
19
Fiscal Year
1998
Total Cost
Indirect Cost

  Institution

Name
University of California Irvine
Department
Type
DUNS #
161202122
City
Irvine
State
CA
Country
United States
Zip Code
92697

  Publications

Paugh, Jerry R; Alfonso-Garcia, Alba; Nguyen, Andrew Loc et al. (2018) Characterization of expressed human meibum using hyperspectral stimulated Raman scattering microscopy. Ocul Surf :
Verdel, Nina; Lentsch, Griffin; Balu, Mihaela et al. (2018) Noninvasive assessment of skin structure by combined photothermal radiometry and optical spectroscopy: coregistration with multiphoton microscopy. Appl Opt 57:D117-D122
Friedman, Jacob E; Dobrinskikh, Evgenia; Alfonso-Garcia, Alba et al. (2018) Pyrroloquinoline quinone prevents developmental programming of microbial dysbiosis and macrophage polarization to attenuate liver fibrosis in offspring of obese mice. Hepatol Commun 2:313-328
Kennedy, Gordon T; Lentsch, Griffin R; Trieu, Brandon et al. (2017) Solid tissue simulating phantoms having absorption at 970 nm for diffuse optics. J Biomed Opt 22:76013
Takesh, Thair; Sargsyan, Anik; Lee, Matthew et al. (2017) Evaluating the Whitening and Microstructural Effects of a Novel Whitening Strip on Porcelain and Composite Dental Materials. Dentistry (Sunnyvale) 7:
Jonscher, Karen R; Stewart, Michael S; Alfonso-Garcia, Alba et al. (2017) Early PQQ supplementation has persistent long-term protective effects on developmental programming of hepatic lipotoxicity and inflammation in obese mice. FASEB J 31:1434-1448
Alfonso-García, Alba; Paugh, Jerry; Farid, Marjan et al. (2017) A machine learning framework to analyze hyperspectral stimulated Raman scattering microscopy images of expressed human meibum. J Raman Spectrosc 48:803-812
Takesh, Thair; Sargsyan, Anik; Anbarani, Afarin et al. (2017) Effects of a Novel Whitening Formulation on Dental Enamel. Dentistry (Sunnyvale) 7:
Malacrida, Leonel; Astrada, Soledad; Briva, Arturo et al. (2016) Spectral phasor analysis of LAURDAN fluorescence in live A549 lung cells to study the hydration and time evolution of intracellular lamellar body-like structures. Biochim Biophys Acta 1858:2625-2635
Choi, Bernard; Tan, Wenbin; Jia, Wangcun et al. (2016) The Role of Laser Speckle Imaging in Port-Wine Stain Research: Recent Advances and Opportunities. IEEE J Sel Top Quantum Electron 2016:

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