Cocrystal of RNA polymerase II/DNA has been recently obtained with diffraction to 5 w on rotating-anode X-ray source. In view of the significant improvement from 5 w resolution with rotating-anode X-ray to 3 w at the synchrotron source in a parallel study of the polymerase alone, the cocrystal is expected to diffract to 3 w with synchrotron beam as well. A cryosoaking procedure has already been developed, making data collection from one crystal feasible. We now propose to collect high resolution X-ray diffraction data on polymerase II/DNA cocrystal as well as its heavy atom derivatives. The goal is to obtain high quality diffraction data for determining the crystal structure of RNA polymerase II/DNA complex.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001209-17
Application #
5222796
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
17
Fiscal Year
1996
Total Cost
Indirect Cost
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