Abstract

Our study last year showed that annexin forms Ca -promoted oligomers. We have now examined the effects of a larger variation in solution condition, in particular the dependence of the oligomerization on pH and ionic strength. We observed that the annexin monomer undergoes a significant conformational change in the absence of Ca in the pH range 6 to 8, which is a physiologically very important range. The expanded form of annexin XII at lower pH seems to be more capable of forming oligomers in a Ca -dependent manner while the compact form at higher pH shows less tendency to form oligomers. At pH 6.3, the oligomer appears to be hexamer. A time-resolved scattering study on the Ca -promoted hexamer formation was conducted. However, the rate of oligomerization appeared to be too fast to record with significant statistics, given the limited sample availability.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001209-17
Application #
5222859
Study Section
Project Start
Project End
Budget Start
Budget End
Support Year
17
Fiscal Year
1996
Total Cost
Indirect Cost

  Publications

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Pluvinage, Benjamin; Grondin, Julie M; Amundsen, Carolyn et al. (2018) Molecular basis of an agarose metabolic pathway acquired by a human intestinal symbiont. Nat Commun 9:1043
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Yoshizawa, Takuya; Ali, Rustam; Jiou, Jenny et al. (2018) Nuclear Import Receptor Inhibits Phase Separation of FUS through Binding to Multiple Sites. Cell 173:693-705.e22
Hettle, Andrew; Fillo, Alexander; Abe, Kento et al. (2017) Properties of a family 56 carbohydrate-binding module and its role in the recognition and hydrolysis of ?-1,3-glucan. J Biol Chem 292:16955-16968
Oberthuer, Dominik; Knoška, Juraj; Wiedorn, Max O et al. (2017) Double-flow focused liquid injector for efficient serial femtosecond crystallography. Sci Rep 7:44628

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