The NG GTPase domain of Ffh, the bacterial homolog of the signal sequence recognition protein SRP54, reveals several unique features that should contribute to our understanding of GTPase structure-function in general. Crystals of the apo-form of the domain diffract to beyond 1.0 E resolution at a synchrotron X-ray source (SSRL BL 7-1), and crystals of the GDP-bound domain diffract almost as well. During previous work we have developed methods for efficient data collection from these crystals to that resolution. Our efforts are now directed at obtaining complete well measured data to ultra-high resolution from both crystal forms.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001209-20
Application #
6119492
Study Section
Project Start
1999-03-01
Project End
2000-04-14
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
20
Fiscal Year
1999
Total Cost
Indirect Cost
Name
Stanford University
Department
Type
DUNS #
800771545
City
Stanford
State
CA
Country
United States
Zip Code
94305
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