With the high resolution fluorescence spectrometers (for Mn and Fe) developed in this lab, we have obtained the basic knowledge of the chemical environments of the Mn and Fe model systems as well as refined the spectrometer. The information revealed from the kb fluorescence dependence on the spin states, oxidation states and ligand field strength can be used for studying more complicated protein systems. We would like to apply this new technique to the Photo System II in order to determine the electronic structures of the intermediate states and to nitrogenase to study the active sites and the interactions between them. We would also like to have a chance to develop a spectrometer for Mo, which will help to solve the puzzle of how the Mo functions during the catalytic process in nitrogenase.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001209-23
Application #
6586706
Study Section
Project Start
2002-03-01
Project End
2003-02-28
Budget Start
Budget End
Support Year
23
Fiscal Year
2002
Total Cost
$143,176
Indirect Cost
Name
Stanford University
Department
Type
DUNS #
800771545
City
Stanford
State
CA
Country
United States
Zip Code
94305
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