A preliminary solution x-ray scattering study was conducted on the pH dependent dimerization of GART. The dimerization process had been studied by other techniques such as UV difference spectroscopy, tyr/trp fluorescence, and size exclusion column chromatography. The solution scattering results confirm that GART is monomeric at pH 8.0 and that it undergoes dimerization at pH 6.2. This helps understand the catalytic function of the protein which is considered to be regulated via the monomer-dimer equilibrium. We will use the solution scattering technique to study the solution structure of the protein in the presence of inhibitors and ligands.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
3P41RR001209-23S1
Application #
6658745
Study Section
Project Start
2002-03-01
Project End
2003-02-28
Budget Start
Budget End
Support Year
23
Fiscal Year
2002
Total Cost
$143,176
Indirect Cost
Name
Stanford University
Department
Type
DUNS #
800771545
City
Stanford
State
CA
Country
United States
Zip Code
94305
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