This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. XAS will be used to characterize the structure of a variety of heavy metals bound to de novo designed peptides. In most cases, XAS is the only method available to provide direct structural information for the metal-site. The immediate goal of this work is to define the relative importance of the different determinants of metal-site structure - i.e., metal-specific stereochemical preference vs. peptide-enforced structural preferences. The ultimate goal of this work is to develop a detailed understanding of the factors involved in controlling metalloprotein metal-specificity, particularly as it relates to metal-ion homeostsis and heavy-metal toxicity.
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