This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Plastic capillaries of identical size produced from six different clear grades of polymers were tested to determine which polymer produces the smallest x-ray background and is the most suitable for the production of sample containers used for protein crystallography x-ray diffraction data collection at cryogenic temperatures. The polymers tested were: Acrylonitrile butadiene styrene (ABS), Polymethyl methacrylate (Acrylic), Polyamide (PA), High Impact Polystyrene (HIP), Polycarbonate (PC), and Low Density Polyethylene (LPDE). Data were collected on SSRL BL7-1. Each sample was exposed to 0.976 A x-rays for about 30 seconds at a temperature of 100 K. The incident intensity was monitored and the exposure time varied to ensure each sample received an equivalent x-ray dose. Care was taken to expose the same volume of plastic from each sample tested. Each plastic was rotated five degrees during the exposure. The scattered intensity was measured by a Q315 CCD detector located at a distance of 251 mm from the sample giving a maximum resolution of 1.37 A. The optical properties of each of the plastic containers were also assessed. This work was done in collaboration with researchers at the Hauptman Woodward Medical Research Institute with the goal of developing a small plastic capillary that can be used both for crystal growth and x-ray diffraction quality screening. The capillary will be used as a pipette tip with both manual pipetters and automated liquid handling robots enabling solutions for crystal growth to be easily drawn inside. For x-ray data collection, the larger end of the capillaries fits onto a metal base and the assembly fits inside the SSRL cassette or uni-puck sample storage container and is compatible with the SSRL automated sample mounting system for automated screening and remote access data collection.

National Institute of Health (NIH)
National Center for Research Resources (NCRR)
Biotechnology Resource Grants (P41)
Project #
Application #
Study Section
Special Emphasis Panel (ZRG1-BPC-E (40))
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
Stanford University
Schools of Arts and Sciences
United States
Zip Code
Vickers, Chelsea; Liu, Feng; Abe, Kento et al. (2018) Endo-fucoidan hydrolases from glycoside hydrolase family 107 (GH107) display structural and mechanistic similarities to ?-l-fucosidases from GH29. J Biol Chem 293:18296-18308
Nguyen, Phong T; Lai, Jeffrey Y; Lee, Allen T et al. (2018) Noncanonical role for the binding protein in substrate uptake by the MetNI methionine ATP Binding Cassette (ABC) transporter. Proc Natl Acad Sci U S A 115:E10596-E10604
Aleman, Fernando; Tzarum, Netanel; Kong, Leopold et al. (2018) Immunogenetic and structural analysis of a class of HCV broadly neutralizing antibodies and their precursors. Proc Natl Acad Sci U S A 115:7569-7574
Herrera, Nadia; Maksaev, Grigory; Haswell, Elizabeth S et al. (2018) Elucidating a role for the cytoplasmic domain in the Mycobacterium tuberculosis mechanosensitive channel of large conductance. Sci Rep 8:14566
Lal, Neeraj K; Nagalakshmi, Ugrappa; Hurlburt, Nicholas K et al. (2018) The Receptor-like Cytoplasmic Kinase BIK1 Localizes to the Nucleus and Regulates Defense Hormone Expression during Plant Innate Immunity. Cell Host Microbe 23:485-497.e5
Pluvinage, Benjamin; Grondin, Julie M; Amundsen, Carolyn et al. (2018) Molecular basis of an agarose metabolic pathway acquired by a human intestinal symbiont. Nat Commun 9:1043
Beyerlein, Kenneth R; J├Ânsson, H Olof; Alonso-Mori, Roberto et al. (2018) Ultrafast nonthermal heating of water initiated by an X-ray Free-Electron Laser. Proc Natl Acad Sci U S A 115:5652-5657
Yoshizawa, Takuya; Ali, Rustam; Jiou, Jenny et al. (2018) Nuclear Import Receptor Inhibits Phase Separation of FUS through Binding to Multiple Sites. Cell 173:693-705.e22
Morrison, Christine N; Spatzal, Thomas; Rees, Douglas C (2017) Reversible Protonated Resting State of the Nitrogenase Active Site. J Am Chem Soc 139:10856-10862
Zhang, Haonan; Qiao, Anna; Yang, Dehua et al. (2017) Structure of the full-length glucagon class B G-protein-coupled receptor. Nature 546:259-264

Showing the most recent 10 out of 604 publications