This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Myelin is the complex structure of multiple phospholipidic membranes that surrounds the axons in the nervous systems. In our laboratory we are interested in defining the atomic structure of compacted myelin. In the central nervous system myelin is formed mainly by two proteins, myelin basic protein (MBP) and proteolipid protein (PLP);at the same time, claudin-11 forms tight junctions that electrically seal the edges of the Ranvier nodules. PLP and claudin-11 are integral membrane proteins, while MBP is a cytoplasmic protein. Solving the unknown atomic structure of the three proteins by Se-Met MAD, we aim to understand the vital packing of membranes that it is compromised when the autoimmune multiple-sclerosis disease emerges. In another line of research, we are studying the structure of the tumor suppressor p53, which is mutated in 50% of all the cancer cases because its central role in the cellular response to DNA damage. We are focusing in the transcription regulation domain which structure is unknown. By phosphorylation, acetylation, methylation and ubiquitination of this domain the activity of p53 is regulated. Initially, we are trying to define its atomic structure in the absence of any modification, to later understand how the different modification affect its regulatory cycle.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001209-30
Application #
7954503
Study Section
Special Emphasis Panel (ZRG1-BPC-E (40))
Project Start
2009-03-01
Project End
2010-02-28
Budget Start
2009-03-01
Budget End
2010-02-28
Support Year
30
Fiscal Year
2009
Total Cost
$618
Indirect Cost
Name
Stanford University
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
009214214
City
Stanford
State
CA
Country
United States
Zip Code
94305
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