The appropriate model contains processes for influx into and efflux from both red blood cells and hepatocytes, as well as hepatocellular sequestration; however, in this case, part of the tracer emerges as labelled bicarbonate formed by the action of pyruvate dehydrogenase, whereas a presumably smaller part is incorporated into other metabolites by the action of pyruvate carboxylase. The model thus incorporates the formation of labelled bicarbonate as a metabolic product. The behavior of bicarbonate in the liver has previously been investigated using the multiple indicator dilution technique. Preliminary evaluation of the data showed that a substantial proportion of blood lactate is carried within red cells and therefore less readily available for hepatic uptake. The situation is similar to that for acetaminophen and will be treated in the same fashion. New experiments were therefore recently performed where lactate was injected in plasma alone (no red cells); the hematocrit in the bolus was re-established by simultaneously injecting blood where the hematocrit was increased by adding 51Cr-labelled red cells (non-pre-equilibrated case), as previously done with thiourea.
| Bassingthwaighte, James B; Butterworth, Erik; Jardine, Bartholomew et al. (2012) Compartmental modeling in the analysis of biological systems. Methods Mol Biol 929:391-438 |
| Dash, Ranjan K; Bassingthwaighte, James B (2010) Erratum to: Blood HbO2 and HbCO2 dissociation curves at varied O2, CO2, pH, 2,3-DPG and temperature levels. Ann Biomed Eng 38:1683-701 |
| Bassingthwaighte, James B; Raymond, Gary M; Butterworth, Erik et al. (2010) Multiscale modeling of metabolism, flows, and exchanges in heterogeneous organs. Ann N Y Acad Sci 1188:111-20 |
| Dash, Ranjan K; Bassingthwaighte, James B (2006) Simultaneous blood-tissue exchange of oxygen, carbon dioxide, bicarbonate, and hydrogen ion. Ann Biomed Eng 34:1129-48 |
| Dash, Ranjan K; Bassingthwaighte, James B (2004) Blood HbO2 and HbCO2 dissociation curves at varied O2, CO2, pH, 2,3-DPG and temperature levels. Ann Biomed Eng 32:1676-93 |
| Kellen, Michael R; Bassingthwaighte, James B (2003) Transient transcapillary exchange of water driven by osmotic forces in the heart. Am J Physiol Heart Circ Physiol 285:H1317-31 |
| Kellen, Michael R; Bassingthwaighte, James B (2003) An integrative model of coupled water and solute exchange in the heart. Am J Physiol Heart Circ Physiol 285:H1303-16 |
| Wang, C Y; Bassingthwaighte, J B (2001) Capillary supply regions. Math Biosci 173:103-14 |
| Swanson, K R; True, L D; Lin, D W et al. (2001) A quantitative model for the dynamics of serum prostate-specific antigen as a marker for cancerous growth: an explanation for a medical anomaly. Am J Pathol 158:2195-9 |
| Swanson, K R; Alvord Jr, E C; Murray, J D (2000) A quantitative model for differential motility of gliomas in grey and white matter. Cell Prolif 33:317-29 |
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