A 'caged' biotin molecule is used to pattern proteins onto a waveguide surface. Upon irradiation with the appropriate ultraviolet light, the caging group is removed and the biotin becomes active.It can then be used to bind streptavidin and subsequently biotinylated antibodies for immunoassay. SIMS is being employed to demonstrate that the caging group has been removed, and to image the patterned surface by identifying regions of bound streptavidin.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001296-14
Application #
6119940
Study Section
Project Start
1998-07-01
Project End
1999-09-27
Budget Start
1997-10-01
Budget End
1998-09-30
Support Year
14
Fiscal Year
1998
Total Cost
Indirect Cost
Name
University of Washington
Department
Type
DUNS #
135646524
City
Seattle
State
WA
Country
United States
Zip Code
98195
Tyler, Bonnie J; Peterson, Richard E (2013) Dead-time correction for time-of-flight secondary-ion mass spectral images: a critical issue in multivariate image analysis. Surf Interface Anal 45:475-478
Tyler, B J; Bruening, C; Rangaranjan, S et al. (2011) TOF-SIMS imaging of adsorbed proteins on topographically complex surfaces with Bi(3) (+) primary ions. Biointerphases 6:135
Medzihradszky, Katalin F (2008) Characterization of site-specific N-glycosylation. Methods Mol Biol 446:293-316
Medzihradszky, Katalin F (2005) Peptide sequence analysis. Methods Enzymol 402:209-44
Sanders, Joan E; Lamont, Sarah E; Karchin, Ari et al. (2005) Fibro-porous meshes made from polyurethane micro-fibers: effects of surface charge on tissue response. Biomaterials 26:813-8
Medzihradszky, Katalin F (2005) In-solution digestion of proteins for mass spectrometry. Methods Enzymol 405:50-65
Medzihradszky, Katalin F (2005) Characterization of protein N-glycosylation. Methods Enzymol 405:116-38
Martin, Stephanie M; Schwartz, Jeffrey L; Giachelli, Cecilia M et al. (2004) Enhancing the biological activity of immobilized osteopontin using a type-1 collagen affinity coating. J Biomed Mater Res A 70:10-9
Cheng, Xuanhong; Wang, Yanbing; Hanein, Yael et al. (2004) Novel cell patterning using microheater-controlled thermoresponsive plasma films. J Biomed Mater Res A 70:159-68
Wagner, Victoria E; Koberstein, Jeffrey T; Bryers, James D (2004) Protein and bacterial fouling characteristics of peptide and antibody decorated surfaces of PEG-poly(acrylic acid) co-polymers. Biomaterials 25:2247-63

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