Abstract

This study aims to determine whether human monocytes/macrophages cultured on pyrolytic carbon (PYC) can oxidize the surface. Oxidation of the pyrolitic carbon is achieved by a number of methods, and surface analytical methods are employed to compare the surfaces so obtained to those observed after cell adhesion studies have been performed. SIMS was employed to characterize the prepared surface as well as to determine how successful various cleaning strategies are in removing adherent proteins and cells, which is necessary for subsequent sample analysis.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001296-17
Application #
6492614
Study Section
Project Start
2001-09-01
Project End
2002-08-31
Budget Start
Budget End
Support Year
17
Fiscal Year
2001
Total Cost
Indirect Cost

  Institution

Name
University of Washington
Department
Type
DUNS #
135646524
City
Seattle
State
WA
Country
United States
Zip Code
98195

  Publications

Tyler, Bonnie J; Peterson, Richard E (2013) Dead-time correction for time-of-flight secondary-ion mass spectral images: a critical issue in multivariate image analysis. Surf Interface Anal 45:475-478
Tyler, B J; Bruening, C; Rangaranjan, S et al. (2011) TOF-SIMS imaging of adsorbed proteins on topographically complex surfaces with Bi(3) (+) primary ions. Biointerphases 6:135
Medzihradszky, Katalin F (2008) Characterization of site-specific N-glycosylation. Methods Mol Biol 446:293-316
Medzihradszky, Katalin F (2005) Peptide sequence analysis. Methods Enzymol 402:209-44
Sanders, Joan E; Lamont, Sarah E; Karchin, Ari et al. (2005) Fibro-porous meshes made from polyurethane micro-fibers: effects of surface charge on tissue response. Biomaterials 26:813-8
Medzihradszky, Katalin F (2005) In-solution digestion of proteins for mass spectrometry. Methods Enzymol 405:50-65
Medzihradszky, Katalin F (2005) Characterization of protein N-glycosylation. Methods Enzymol 405:116-38
Wagner, Victoria E; Koberstein, Jeffrey T; Bryers, James D (2004) Protein and bacterial fouling characteristics of peptide and antibody decorated surfaces of PEG-poly(acrylic acid) co-polymers. Biomaterials 25:2247-63
Tsai, W B; Shi, Q; Grunkemeier, J M et al. (2004) Platelet adhesion to radiofrequency glow-discharge-deposited fluorocarbon polymers preadsorbed with selectively depleted plasmas show the primary role of fibrinogen. J Biomater Sci Polym Ed 15:817-40
Martin, Stephanie M; Schwartz, Jeffrey L; Giachelli, Cecilia M et al. (2004) Enhancing the biological activity of immobilized osteopontin using a type-1 collagen affinity coating. J Biomed Mater Res A 70:10-9

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