In this project the Matrix Assisted Laser Desorption Ionization Mass Spectrometry (MALDI) concept is being extended for use in Time-of-Flight Static Secondary Ion Mass Spectrometry (ToF-SSIMS). The overall aim of the project is to use MALDI matrix molecules to increase both the ion yields of intact proteins and peptides and to reduce fragmentation caused by Cs+ ion beam impact that occurs in standard SIMS analysis. Initial work is directed towards the detection of intact model peptide molecules. Model peptides with ?-sheet (YGLKLKLKL-NH2 Mw=1075.42 amu) and ?-helix (YG(LKKLLKL)2-NH2 Mw=1911.34 amu)) tertiary structures (synthesized using standard FMOC solid phase synthesis) are being used in conjunction with standard MALDI matrices [2,5-dihydroxybenzoic acid (DHB) and 3,5-dimethoxy-4-hydroxycinnamic (sinapinic acid (SA))] to facilitate generation of molecular ions upon irradiation with the SIMS Cs+ ion >source. SIMS imaging with micrometer spatial resolution of mat rix/peptid es crystals on Si wafers has to date suggested that this mode of analysis can be applied to specifically map the distribution of peptide molecules within MALDI crystals.

National Institute of Health (NIH)
National Center for Research Resources (NCRR)
Biotechnology Resource Grants (P41)
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University of Washington
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