Recent studies with endothelial cells and epithelial cells show that denial of anchorage causes apoptosis whereas fibroblasts were unaffected. In our laboratory, however, we find that a combination of growth factor withdrawal and denial of anchorage induces apoptosis in human diploid fibroblasts. We propose that the two signals are required for apoptosis and that one signal alone leads to cellular senescence rather than apoptosis. Using recently developed FCM and biochemical techniques we will define the signal transduction pathways that lead to cellular senescence and apoptosis in human diploid fibroblasts. The final goal of this project is to investigate how denial of anchorage is related to the regulation of the cell cycle.
| Frumkin, Jesse P; Patra, Biranchi N; Sevold, Anthony et al. (2016) The interplay between chromosome stability and cell cycle control explored through gene-gene interaction and computational simulation. Nucleic Acids Res 44:8073-85 |
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| Ai, Ye; Sanders, Claire K; Marrone, Babetta L (2013) Separation of Escherichia coli bacteria from peripheral blood mononuclear cells using standing surface acoustic waves. Anal Chem 85:9126-34 |
| Sanders, Claire K; Mourant, Judith R (2013) Advantages of full spectrum flow cytometry. J Biomed Opt 18:037004 |
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| Vuyisich, Momchilo; Sanders, Claire K; Graves, Steven W (2012) Binding and cell intoxication studies of anthrax lethal toxin. Mol Biol Rep 39:5897-903 |
| Chaudhary, Anu; Ganguly, Kumkum; Cabantous, Stephanie et al. (2012) The Brucella TIR-like protein TcpB interacts with the death domain of MyD88. Biochem Biophys Res Commun 417:299-304 |
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