Bacterial endotoxins are important modulators of septic shock. We have examined for the first time the pathway of LPS action on neutrophils in blood. This pathway involves a plasma binding protein and a cell surface receptor CD14. We have shown that LPS can act directly upon the neutrophil in blood without the production of inflammatory mediators by other cell types. These studies set the stage for detailed mechanistic evaluation of the action of inflammatory mediators and regulators in physiological systems. LPS causes the modulation of important adhesive proteins on leukocyte-selectins and integrins which alter the ability of neutrophils to recognize one another and alter their compartmentation in the circulation. Ongoing studies are aimed at defining the relationship between the expression of adhesion molecules and neutrophil physiology.
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| Chaudhary, Anu; Ganguly, Kumkum; Cabantous, Stephanie et al. (2012) The Brucella TIR-like protein TcpB interacts with the death domain of MyD88. Biochem Biophys Res Commun 417:299-304 |
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