We have developed a stopped-flow flow cytometer with subsecond resolution to be used in kinetic analysis of cell activation. This instrument is extending the application of flow cytometry to important biological molecules that interact with cell surface receptors. The instrument is expected to make it possible to examine the interaction of hormones and other small molecules with receptors when the affinities of the binding are too low to be examined by other methods. We have modified a commercial stepper-motor-driven, stopped-flow mixing system, and integrated the mixer with a flow cytometer. This device is being used to detect ligand-receptor interactions in living cells and is expected to extend the use of flow cytometers to new communities of biomedical and health scientists. In the previous year we improved the system by decreasing the dead volume between the exit valve of the delivery system and the port of the flow system so as to achieve delivery times under 300 msces. A description of the device has been published (Nolan et al, Cytometry 1995) We have recently modified the mixing device to permit two sequential mixing operations and added another phototube tto the cytometer which will allow analysis of the complex time-dependent biological processes that occur during the activation of living cells. Measurements are being made on cells expressing ligands of diffrerent afifinities and mutant receptors.
| Frumkin, Jesse P; Patra, Biranchi N; Sevold, Anthony et al. (2016) The interplay between chromosome stability and cell cycle control explored through gene-gene interaction and computational simulation. Nucleic Acids Res 44:8073-85 |
| Johnson, Leah M; Gao, Lu; Shields IV, C Wyatt et al. (2013) Elastomeric microparticles for acoustic mediated bioseparations. J Nanobiotechnology 11:22 |
| Micheva-Viteva, Sofiya N; Shou, Yulin; Nowak-Lovato, Kristy L et al. (2013) c-KIT signaling is targeted by pathogenic Yersinia to suppress the host immune response. BMC Microbiol 13:249 |
| Ai, Ye; Sanders, Claire K; Marrone, Babetta L (2013) Separation of Escherichia coli bacteria from peripheral blood mononuclear cells using standing surface acoustic waves. Anal Chem 85:9126-34 |
| Sanders, Claire K; Mourant, Judith R (2013) Advantages of full spectrum flow cytometry. J Biomed Opt 18:037004 |
| Cushing, Kevin W; Piyasena, Menake E; Carroll, Nick J et al. (2013) Elastomeric negative acoustic contrast particles for affinity capture assays. Anal Chem 85:2208-15 |
| Piyasena, Menake E; Austin Suthanthiraraj, Pearlson P; Applegate Jr, Robert W et al. (2012) Multinode acoustic focusing for parallel flow cytometry. Anal Chem 84:1831-9 |
| Austin Suthanthiraraj, Pearlson P; Piyasena, Menake E; Woods, Travis A et al. (2012) One-dimensional acoustic standing waves in rectangular channels for flow cytometry. Methods 57:259-71 |
| Vuyisich, Momchilo; Sanders, Claire K; Graves, Steven W (2012) Binding and cell intoxication studies of anthrax lethal toxin. Mol Biol Rep 39:5897-903 |
| Chaudhary, Anu; Ganguly, Kumkum; Cabantous, Stephanie et al. (2012) The Brucella TIR-like protein TcpB interacts with the death domain of MyD88. Biochem Biophys Res Commun 417:299-304 |
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