Using the fourth generation language IDL (Interactive Data Language - Research Systems, Inc., Boulder, CO) we have developed an elaborate program """"""""IDLYK"""""""" for the display and analysis of flow data. This program has many functions and capabilities. First and foremost, IDLYK lets the users of our LACEL based cytometers convert their data files from the LACEL format - developed in the early 1970's by Gary Salzman and Jim Jett - to the now widely accepted FCS format standard. This permits the use of other commercial software packages for special purposes such as cell-cycle analysis and intricate immunophenotyping. Other features include: a neural net clustering algorithm (in collaboration with Mark Naivar), and many unique display formats: image display, line-plot, shaded surfaces, and three dimensional histograms as dotplots or clouds. Additionally, I have added Kinetic data display and processing, which is used extensively by John Nolan. The software has been utilized during the last 5-6 years by numerous investigators in Life Sciences: Donna Gadbois, Joe D'Anna, Mira Bussod, Nancy Lehnert, and various people in Harry Crissman's lab. It is also currently being used extensively in the labs of Janis Georgie at UCLA and Chris Eichman at Smith-Kline, Beecham Pharmaceuticals in King of Prussia, PA.

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Los Alamos National Lab
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Frumkin, Jesse P; Patra, Biranchi N; Sevold, Anthony et al. (2016) The interplay between chromosome stability and cell cycle control explored through gene-gene interaction and computational simulation. Nucleic Acids Res 44:8073-85
Ai, Ye; Sanders, Claire K; Marrone, Babetta L (2013) Separation of Escherichia coli bacteria from peripheral blood mononuclear cells using standing surface acoustic waves. Anal Chem 85:9126-34
Sanders, Claire K; Mourant, Judith R (2013) Advantages of full spectrum flow cytometry. J Biomed Opt 18:037004
Cushing, Kevin W; Piyasena, Menake E; Carroll, Nick J et al. (2013) Elastomeric negative acoustic contrast particles for affinity capture assays. Anal Chem 85:2208-15
Johnson, Leah M; Gao, Lu; Shields IV, C Wyatt et al. (2013) Elastomeric microparticles for acoustic mediated bioseparations. J Nanobiotechnology 11:22
Micheva-Viteva, Sofiya N; Shou, Yulin; Nowak-Lovato, Kristy L et al. (2013) c-KIT signaling is targeted by pathogenic Yersinia to suppress the host immune response. BMC Microbiol 13:249
Piyasena, Menake E; Austin Suthanthiraraj, Pearlson P; Applegate Jr, Robert W et al. (2012) Multinode acoustic focusing for parallel flow cytometry. Anal Chem 84:1831-9
Austin Suthanthiraraj, Pearlson P; Piyasena, Menake E; Woods, Travis A et al. (2012) One-dimensional acoustic standing waves in rectangular channels for flow cytometry. Methods 57:259-71
Vuyisich, Momchilo; Sanders, Claire K; Graves, Steven W (2012) Binding and cell intoxication studies of anthrax lethal toxin. Mol Biol Rep 39:5897-903
Chaudhary, Anu; Ganguly, Kumkum; Cabantous, Stephanie et al. (2012) The Brucella TIR-like protein TcpB interacts with the death domain of MyD88. Biochem Biophys Res Commun 417:299-304

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