Adhesive interactions among leukocytes and between leukocytes and endothelium are central to processes of inflammation. These interactions are mediated by sequential molecular steps involving carbohydrates and selectins, and integrins and ICAMs. We used flow cytometry to examine the interaction of neutrophils with cells transfected with ICAM, a counter structure for the neutrophil integrins. A manuscript has been submitted (Simon). We extended this approach to examine the mechanisms of aggregation of lymphoma cells which may contribute to leukostasis. A manuscript has been published in Blood (Larson) In Year 16, we have begun to compare selectins of different lengths (McEver and Edwards) for their ability to support cell adhesion under conditions of defined shear using adhesion produced in a cone and plate visoometer and flow cytometric analysis. We have also begun to examine the binding of soluble selectins to native cell surface mucins. Pilot data suggest that the dissociation of soluble P-slelectin from the mucin PSGL occurs on the second time frame. Bruce Edwards has become affiliated with the NFCR by joining the faculty at UNM. A new project is being developed to use oligonucleotide ligands to selectins (Parma) to study the activity and topography of cell surface adhesion receptors with resonance energy transfer and rapid mix flow cytometry.
| Frumkin, Jesse P; Patra, Biranchi N; Sevold, Anthony et al. (2016) The interplay between chromosome stability and cell cycle control explored through gene-gene interaction and computational simulation. Nucleic Acids Res 44:8073-85 |
| Johnson, Leah M; Gao, Lu; Shields IV, C Wyatt et al. (2013) Elastomeric microparticles for acoustic mediated bioseparations. J Nanobiotechnology 11:22 |
| Micheva-Viteva, Sofiya N; Shou, Yulin; Nowak-Lovato, Kristy L et al. (2013) c-KIT signaling is targeted by pathogenic Yersinia to suppress the host immune response. BMC Microbiol 13:249 |
| Ai, Ye; Sanders, Claire K; Marrone, Babetta L (2013) Separation of Escherichia coli bacteria from peripheral blood mononuclear cells using standing surface acoustic waves. Anal Chem 85:9126-34 |
| Sanders, Claire K; Mourant, Judith R (2013) Advantages of full spectrum flow cytometry. J Biomed Opt 18:037004 |
| Cushing, Kevin W; Piyasena, Menake E; Carroll, Nick J et al. (2013) Elastomeric negative acoustic contrast particles for affinity capture assays. Anal Chem 85:2208-15 |
| Chaudhary, Anu; Ganguly, Kumkum; Cabantous, Stephanie et al. (2012) The Brucella TIR-like protein TcpB interacts with the death domain of MyD88. Biochem Biophys Res Commun 417:299-304 |
| Marina, Oana C; Sanders, Claire K; Mourant, Judith R (2012) Correlating light scattering with internal cellular structures. Biomed Opt Express 3:296-312 |
| Houston, Jessica P; Naivar, Mark A; Jenkins, Patrick et al. (2012) Capture of Fluorescence Decay Times by Flow Cytometry. Curr Protoc Cytom 59:1.25.1-1.25.21 |
| Marina, Oana C; Sanders, Claire K; Mourant, Judith R (2012) Effects of acetic acid on light scattering from cells. J Biomed Opt 17:085002-1 |
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