Eosinophils and basophils are rare blood cells that play important roles in pulmonary inflammation associated with asthma. We have isolated eosinophils from blood and used flow cytometry to study their adhesive properties with transfected cell lines expressing selectin and integrin adhesion molecules and combinations of them. Initial studies suggest a potential basis for preferential eosinophil accumulation in asthma. We hypothesize that histamine release in asthma upregulates P-selectin on lung vascular endothelium. Our flow cytometry data suggests that eosinophils interact with low levels of P-selectin on cells better than neutrophils.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001315-17
Application #
6120014
Study Section
Project Start
1999-07-01
Project End
2000-06-30
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
17
Fiscal Year
1999
Total Cost
Indirect Cost
Name
Los Alamos National Lab
Department
Type
DUNS #
City
Los Alamos
State
NM
Country
United States
Zip Code
87545
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