Flow cytometry techniques to size individual fragments of DNA and analyze other small biological particles are under development. During the past year, it was discovered that the dye being used has a small dynamic range requiring reasonably accurate knowledge of DNA concentrations prior to staining. A new dye, Pico Green has been found to have a much wider dynamic range alleviating the need to precisely know the DNA concentration prior to analysis. Current efforts are focused on applying the technology to bacterial species and strain identification. Details are contained in the Project 3 progress report and Research Highlight Bacterial Fingerprinting by Flow Cytometry Bacterial Species Discrimination.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001315-19
Application #
6470688
Study Section
Project Start
2001-07-01
Project End
2002-06-30
Budget Start
1998-10-01
Budget End
1999-09-30
Support Year
19
Fiscal Year
2001
Total Cost
$121,168
Indirect Cost
Name
Los Alamos National Lab
Department
Type
DUNS #
City
Los Alamos
State
NM
Country
United States
Zip Code
87545
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