This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.The Huanglongbing (HLB) disease of citrus is one of the most destructive diseases of Citrus species. HLB causes poor quality fruit and a rapid tree decline and is found in Africa, Asia, the Indian subcontinent, the Arabian peninsula and South America. To develop controls, we need to understand the epidemiology of the disease. A prerequisite to this understanding is a reliable method for the identification of the causal pathogen. Since the organism cannot be cultured, molecular-based assays are currently being used for diagnosis. However, these assays are not entirely specific and cross-reactions occur leading to false positive identifications. In order to develop a means to cultivate and detect the HLB pathogen, we want to sequence the pathogen. It is possible to sequence an entire bacterial sequence from a single cell. Using flow cell sorting we will isolate the bacterium from environmental samples for sequencing. With an annotated genome, key metabolic pathways and products for the bacteria can be identified and used to help develop culture methods.
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