This subproject is one of many research subprojects utilizing theresources provided by a Center grant funded by NIH/NCRR. The subproject andinvestigator (PI) may have received primary funding from another NIH source,and thus could be represented in other CRISP entries. The institution listed isfor the Center, which is not necessarily the institution for the investigator.We will expand our ongoing efforts in the development of microsphere-based assays to develop a new approach to the genetic analysis of environmental samples that will enable sensitive and rapid detection of pathogens with a high degree of confidence and a low false alarm rate. Specifically, we will develop multiplex microsphere-based assay kits to provide rapid point detection of pathogen-specific PCR products amplified from environmental samples, allowing screening for a panel of organisms in a single test. Because flow cytometry is the analysis platform, sample preparation is simple, requiring no wash steps, and analysis is rapid, less than one minute. The new assays will be compatible with commercial laboratory flow cytometers as well as smaller fieldable instruments, including the Autonomous Pathogen Detector (APD) being developed as part of the DOE CBNP effort. These new tools will allow flexible design to provide rapid analysis in a variety of response scenarios.

Agency
National Institute of Health (NIH)
Institute
National Center for Research Resources (NCRR)
Type
Biotechnology Resource Grants (P41)
Project #
5P41RR001315-27
Application #
7724227
Study Section
Special Emphasis Panel (ZRG1-CB-K (40))
Project Start
2008-07-01
Project End
2009-03-31
Budget Start
2008-07-01
Budget End
2009-03-31
Support Year
27
Fiscal Year
2008
Total Cost
$16,102
Indirect Cost
Name
Los Alamos National Lab
Department
Type
DUNS #
175252894
City
Los Alamos
State
NM
Country
United States
Zip Code
87545
Frumkin, Jesse P; Patra, Biranchi N; Sevold, Anthony et al. (2016) The interplay between chromosome stability and cell cycle control explored through gene-gene interaction and computational simulation. Nucleic Acids Res 44:8073-85
Johnson, Leah M; Gao, Lu; Shields IV, C Wyatt et al. (2013) Elastomeric microparticles for acoustic mediated bioseparations. J Nanobiotechnology 11:22
Micheva-Viteva, Sofiya N; Shou, Yulin; Nowak-Lovato, Kristy L et al. (2013) c-KIT signaling is targeted by pathogenic Yersinia to suppress the host immune response. BMC Microbiol 13:249
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Sanders, Claire K; Mourant, Judith R (2013) Advantages of full spectrum flow cytometry. J Biomed Opt 18:037004
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Chaudhary, Anu; Ganguly, Kumkum; Cabantous, Stephanie et al. (2012) The Brucella TIR-like protein TcpB interacts with the death domain of MyD88. Biochem Biophys Res Commun 417:299-304
Marina, Oana C; Sanders, Claire K; Mourant, Judith R (2012) Correlating light scattering with internal cellular structures. Biomed Opt Express 3:296-312
Houston, Jessica P; Naivar, Mark A; Jenkins, Patrick et al. (2012) Capture of Fluorescence Decay Times by Flow Cytometry. Curr Protoc Cytom 59:1.25.1-1.25.21
Marina, Oana C; Sanders, Claire K; Mourant, Judith R (2012) Effects of acetic acid on light scattering from cells. J Biomed Opt 17:085002-1

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