): The thrust of the project is to develop and employ ground-breaking methods in the use of infrared spectroscopy for the study of biological macromolecules. Developments will include infrared analogues of NMR spectroscopy for the determination of the dynamics of structure change; heterodyned 2D and 3D IR methods for study of amide I and N-H stretch modes of proteins; pump/probe methods with IR pulses, and methods to determine energy transport between remote C=O and N-H or O-H groups in peptides and proteins; methods to measure and interpret IR photon echoes and vibrational frequency correlation functions essential for understanding the response functions needed for 2D IR; instrumentation to advance T-jumps associated with IR, and of mixing kinetic methods by multiplexing infrared detection; T-jumps and mixing methods using IR probes of the intermediates; immobilization techniques to examine equilibrium fluctuations and kinetics of proteins, and other assemblies such as cells, using single-molecule fluorescence methods; methods to study ultrafast light-induced processes in biological systems probed by optical and infrared methods. There are numerous collaborative projects designed to push the technologies being developed. These involve studies on reaction centers, rhodopsin and bacteriorhodopsin, the ribosome and t-RNA, LH2 single assemblies and terahertz band-to-band transitions, pentapeptide 2D IR, calmodulin, NO-myoglobin, 310 helicies, single coiled-coils, folding and unfolding of proteins, ?-helix 2D IR, interleukin 5, ?-interferon, single ion channels, cytochrome-c trehalose, prophyrin arrays, nitrile as a probe of conformations, triplet state singles, DNA base sequence probes, and rapid-mixing folding studies. There are service projects proposed in ultrafast optical and infrared, two-dimension and single -molecule spectroscopy, in areas of DNA helicases, protein folding, serpin-serine protease interaction, laser-induced pH juimps, porphyrin electon transfer, chemosensors, helix-coil transitions, ribonucleotide reductase, light-harvesting self assembly, and NorFES single peptide study. The Resource expects to publish two newsletters each year. Besides regular seminars they will hold a synmposium on multidimensional spectroscopies and a workshop in advances in infrared laser spectroscopy . The Resource will train visiting faculty researchers, post doctorals, and graduate and undergraduate students in the applicaitons of lasers to biology.

National Institute of Health (NIH)
National Center for Research Resources (NCRR)
Biotechnology Resource Grants (P41)
Project #
Application #
Study Section
Special Emphasis Panel (ZRG1-MEDB (02))
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of Pennsylvania
Schools of Arts and Sciences
United States
Zip Code
Sheth, Rahul A; Arellano, Ronald S; Uppot, Raul N et al. (2015) Prospective trial with optical molecular imaging for percutaneous interventions in focal hepatic lesions. Radiology 274:917-26
Roussakis, Emmanuel; Spencer, Joel A; Lin, Charles P et al. (2014) Two-photon antenna-core oxygen probe with enhanced performance. Anal Chem 86:5937-45
Courter, Joel R; Abdo, Mohannad; Brown, Stephen P et al. (2014) The design and synthesis of alanine-rich ?-helical peptides constrained by an S,S-tetrazine photochemical trigger: a fragment union approach. J Org Chem 79:759-68
Chuntonov, Lev; Ma, Jianqiang (2013) Quantum process tomography quantifies coherence transfer dynamics in vibrational exciton. J Phys Chem B 117:13631-8
Culik, Robert M; Annavarapu, Srinivas; Nanda, Vikas et al. (2013) Using D-Amino Acids to Delineate the Mechanism of Protein Folding: Application to Trp-cage. Chem Phys 422:
Kuroda, Daniel G; Bauman, Joseph D; Challa, J Reddy et al. (2013) Snapshot of the equilibrium dynamics of a drug bound to HIV-1 reverse transcriptase. Nat Chem 5:174-81
Lam, A R; Moran, S D; Preketes, N K et al. (2013) Study of the ?D-crystallin protein using two-dimensional infrared (2DIR) spectroscopy: experiment and simulation. J Phys Chem B 117:15436-43
Kuroda, Daniel G; Singh, Prabhat K; Hochstrasser, Robin M (2013) Differential hydration of tricyanomethanide observed by time resolved vibrational spectroscopy. J Phys Chem B 117:4354-64
Singh, Prabhat K; Kuroda, Daniel G; Hochstrasser, Robin M (2013) An ion's perspective on the molecular motions of nanoconfined water: a two-dimensional infrared spectroscopy study. J Phys Chem B 117:9775-84
Goldberg, Jacob M; Speight, Lee C; Fegley, Mark W et al. (2012) Minimalist probes for studying protein dynamics: thioamide quenching of selectively excitable fluorescent amino acids. J Am Chem Soc 134:6088-91

Showing the most recent 10 out of 128 publications